Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue Homogenate
Product nameFraction-PREP Cell Fractionation Kit
Sample typeAdherent cells, Suspension cells, Tissue Homogenate
Fraction-PREP Cell Fractionation Kit (ab288085), previously known as FractionPREP™ Cell Fractionation Kit (Cat# K270-50), is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
This product is manufactured by BioVision, an Abcam company and was previously called K270 FractionPREP™ Cell Fractionation Kit. K270-50 is the same size as the 50 test size of ab288085.
Storage instructionsPlease refer to protocols.
Components Identifier 50 tests Cytosol Extraction Buffer (CEB) WM 1 x 20ml DTT (1 M) Blue cap 1 x 150µl Membrane Extraction Buffer-A (MEB-A) WM 1 x 20ml Membrane Extraction Buffer-B (MEB-B) Green cap 1 x 1.2ml Nuclear Extraction Buffer (NEB) NM 1 x 10ml Protease Inhibitor Cocktail Red cap 1 vial
ab288085 has not yet been referenced specifically in any publications.