Product nameAnti-Fragilis antibody
See all Fragilis primary antibodies
DescriptionRabbit polyclonal to Fragilis
Tested applicationsSuitable for: IP, IHC-P, IHC-G, ICC/IF, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Rat
- WB: Mouse Embryonic Stem Cell lysate, Mouse Embryonic Germ Cell lysate IHC-Fr: male genital ridges of E12.5 mouse embryos
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 21615296|
|IHC-G||Use at an assay dependent concentration. PubMed: 22446628|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-Fr||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).|
FunctionIFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.
Sequence similaritiesBelongs to the CD225 family.
modificationsPalmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
Cellular localizationCell membrane.
- Information by UniProt
- 1 8U antibody
- Fragilis antibody
- IFITM3 antibody
IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).
ab15592 (anti-Fragilis) = red
Oct4 = green
DAPI (DNA) = blue
The dilution of ab15592 used was 10 ug/ml.
Anti-Fragilis antibody, ab15592, recognised a small proportion of cells in a culture of human embryonic stem cells differentiated into a heterogenous mix of cells. Based upon the sequence of the immunogen used to generate this antibody the protein it recognizes is likely to be IFITM2 and/or IFITM3.
Fragilis - Primordial Germ Cell Marker was immunoprecipitated using 0.5mg E14Tg2a whole cell extract, 5µg of Rabbit polyclonal to Fragilis and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, E14Tg2a whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15592.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 13kDa: Fragilis - Primordial Germ Cell Marker.
This product has been referenced in:
- Leopardo NP & Vitullo AD Early embryonic development and spatiotemporal localization of mammalian primordial germ cell-associated proteins in the basal rodent Lagostomus maximus. Sci Rep 7:594 (2017). IHC-P ; Other species . Read more (PubMed: 28377629) »
- Zhang XL et al. Integrative epigenomic analysis reveals unique epigenetic signatures involved in unipotency of mouse female germline stem cells. Genome Biol 17:162 (2016). Read more (PubMed: 27465593) »