Question (15810) | Anti-Fragilis antibody (ab15592)

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Question

The question is whether the antibody (ab15592) binds on the surface epitope of Fragilis. In other words, which part of Fragilis is bound with the antibody? For examples, is it a surface epitope, an intramembrane epitope, or an cytosolic epitope? The reason why I ask this question is to know whether I can use this antibody for FACS sorting. Thanks

Answer

Thank you for your enquiry. I have looked at the amino acid sequence used to generate the antibody and can confirm it is at the N terminus of the protein, however the SwissProt reference we currently have (Q9D8L6) does not specify which part of the protein is extracellular, intramembrane or cytosolic so unfortunately I cannot answer your question. If you are aware of the amino acid sequences of each domain I can certainly provide you with the immunogen sequence so you can find this out. I'm sorry I couldn't help you more on this occasion,

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