Recombinant
RabMAb

Anti-Fragilis antibody [EPR5242] (ab109429)

Overview

  • Product name
    Anti-Fragilis antibody [EPR5242]
    See all Fragilis primary antibodies
  • Description
    Rabbit monoclonal [EPR5242] to Fragilis
  • Host species
    Rabbit
  • Specificity
    Predicted cross-reactivity with IFITM2 (93% homology) but this has not been tested.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Fragilis aa 1-100 (internal sequence). The exact sequence is proprietary.

  • Positive control
    • HeLa, 293T, and Human placenta lysates; Human kidney tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR5242
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.
    • Sequence similarities
      Belongs to the CD225 family.
    • Post-translational
      modifications
      Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 8U antibody
      • Fragilis antibody
      • IFITM3 antibody
      • IFM3_HUMAN antibody
      • Interferon induced transmembrane protein 3 (1 8U) antibody
      • Interferon Induced Transmembrane Protein 3 antibody
      • Interferon inducible antibody
      • Interferon inducible protein 1 8U antibody
      • Interferon Inducible Protein 15 antibody
      • Interferon Inducible Protein Homolog antibody
      • Interferon-induced transmembrane protein 3 antibody
      • Interferon-inducible protein 1-8U antibody
      • IP15 antibody
      see all

    Images

    • All lanes : Anti-Fragilis antibody [EPR5242] (ab109429) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 15 kDa

    • Immunohistochemical analysis of Fragilis in paraffin-embedded Human kidney tissue using ab109429 at 1/250 dilution.
    • ICC/IF image of ab109429 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109429 at 1/50 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    This product has been referenced in:
    • Li P  et al. Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions. Biochim Biophys Acta 1860:885-893 (2017). Read more (PubMed: 28511927) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Cow Cell (Blastocyst Day7)
    Permeabilization
    Yes - 0,2% Triton X
    Specification
    Blastocyst Day7
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Irene Röttinger

    Verified customer

    Submitted Nov 30 2016

    Thank you for contacting Abcam.



    The immunogen for cat.# ab109429 has a 93% homology with IFITM2. However, we have not tested any potential cross-reactivity with IFITM2. I would predict cross-reactivity, but it is not guaranteed wit...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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