FRAP Assay Kit (Ferric Reducing Antioxidant Power Assay) (ab234626)

Overview

  • Product name

    FRAP Assay Kit (Ferric Reducing Antioxidant Power Assay)
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Other biological fluids
  • Assay type

    Quantitative
  • Sensitivity

    0.2 mM
  • Product overview

    FRAP Assay Kit ab234626 provides a quick, sensitive and easy way for measuring the antioxidant capacity of various biological samples. FRAP assay stands for Ferric Reducing Antioxidant Power Assay.


    The FRAP assay is high-throughput, adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents.


    The assay measures the antioxidant potential in samples through the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the samples. Following the reduction of the ferric iron, a blue color develops that is read colorimetrically at 594 nm.


    The antioxidant potential of samples is determined using a ferrous iron standard curve and results are expressed as Fe2+ equivalents (µM) or FRAP value.

  • Notes

    Related products

    Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 200 tests
    FeCl3 Solution 1 x 4ml
    Ferrous Standard (2 mM) 1 x 1ml
    FRAP Assay Buffer 1 x 50ml
    FRAP Positive Control 1 x 0.1ml
    FRAP Probe 1 x 4ml

Images

  • Ferrous standard curve.

  • Absorbance readings for positive control and 10 μl diluted solutions of grape juice (1:50 dilution with dH2O), strawberry methanolic extract (extract made from 50 mg of freeze-dried strawberries in 5 ml of extraction solvent and final solution diluted 1:80 times with dH2O) and green tea (brewed for 5 minutes and diluted 1:120 times with dH2O).

  • Absorbance readings for mM Ferrous equivalents or FRAP of grape juice, strawberry and green tea.

Protocols

References

ab234626 has not yet been referenced specifically in any publications.

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