Overview

  • Product name

    Anti-FRAT1 antibody - C-terminal
    See all FRAT1 primary antibodies
  • Description

    Rabbit polyclonal to FRAT1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 216-279 of Human FRAT1 (Uniprot ID: Q92837).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; A431 cells; Human SG xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)

Target

  • Relevance

    The protein encoded by the FRAT1 gene belongs to the GSK-3-binding protein family. The protein inhibits GSK-3-mediated phosphorylation of beta-catenin and also positively regulates the Wnt signaling pathway. It may play a role in tumor progression and in lymphomagenesis.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • FRAT 1 antibody
    • frequently rearranged in advanced T cell lymphomas antibody
    • Frequently rearranged in advanced T-cell lymphomas antibody
    • GSK 3 binding protein FRAT1 antibody
    • proto oncogene FRAT1 antibody
    see all

Images

  • Anti-FRAT1 antibody - C-terminal (ab137391) at 1/1000 dilution + A431 whole cell lysate
    at 30 µg

    Predicted band size: 29 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed A431 cells, labelling FRAT1 with ab137391 at 1/200 dilution. Lower image is costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human SG xenograft tissue, labeling FRAT1 with ab137391 at 1/500 dilution.

References

This product has been referenced in:

  • Zhang W  et al. Frequently rearranged in advanced T-cell lymphomas-1 demonstrates oncogenic properties in prostate cancer. Mol Med Rep 14:3551-8 (2016). IHC-P ; Human . Read more (PubMed: 27599661) »
See 1 Publication for this product

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