Overview

  • Product name

    Anti-FRAT1 antibody [EPR3900]
    See all FRAT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3900] to FRAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FRAT1 aa 100-200 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • HeLa and MCF7 cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
Flow Cyt 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance

      The protein encoded by the FRAT1 gene belongs to the GSK-3-binding protein family. The protein inhibits GSK-3-mediated phosphorylation of beta-catenin and also positively regulates the Wnt signaling pathway. It may play a role in tumor progression and in lymphomagenesis.
    • Cellular localization

      Cytoplasmic
    • Database links

    • Alternative names

      • FRAT 1 antibody
      • frequently rearranged in advanced T cell lymphomas antibody
      • Frequently rearranged in advanced T-cell lymphomas antibody
      • GSK 3 binding protein FRAT1 antibody
      • proto oncogene FRAT1 antibody
      see all

    Images

    • All lanes : Anti-FRAT1 antibody [EPR3900] (ab108405) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    References

    This product has been referenced in:

    • Li B  et al. Network-Wide Screen Identifies Variation of Novel Precise On-Module Targets Using Conformational Modudaoism. CPT Pharmacometrics Syst Pharmacol 7:16-25 (2018). IP . Read more (PubMed: 28925077) »
    • Werner TV  et al. MiR-34a-3p alters proliferation and apoptosis of meningioma cells in vitro and is directly targeting SMAD4, FRAT1 and BCL2. Aging (Albany NY) 9:932-954 (2017). WB . Read more (PubMed: 28340489) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab108405.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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