Overview

  • Product name
    Anti-FRAT2 antibody
  • Description
    Goat polyclonal to FRAT2
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: GPDRIALQPSGSLL, corresponding to C terminal amino acids 220-233 of Human FRAT2.

  • Positive control
    • Human Heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab2470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human FRAT2 peptide (ab22910).

Approx 30-35kDa band seen in Human Heart lysate.

Target

  • Database links
  • Alternative names
    • Frequently rearranged in advanced T-cell lymphomas 2 antibody
    • GSK-3 binding protein FRAT2 antibody

Images

  • ab2470 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2470 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References

ab2470 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

1. We incubate the membrane with the primary antibody for 1 hr, then apply 2 washes and 3 washes after the secondary antibody. We would suggest adding 0.05% Tween20 to the blocking solution. 2.We use ECLPlus (Amersham) rather than ECL. ECLPlus is known to be approx 10X sensitive. 3. We also use PVDF membrane rather than nitrocellulose membrane for maximum efficiency in protein transfer. If you are still having problem with this antibody then please do let us know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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