Anti-Frataxin antibody [18A5DB1] (ab110328)


  • Product name
    Anti-Frataxin antibody [18A5DB1]
    See all Frataxin primary antibodies
  • Description
    Mouse monoclonal [18A5DB1] to Frataxin
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length mature protein, corresponding to amino acids 56-210 of Human Frataxin.

  • Positive control
    • Recombinant Human Frataxin; normal Human fibroblasts (MRC5); HL60 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human colon adenocarcinoma.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.

    Product was previously marketed under the MitoSciences sub-brand.

    Although this antibody does work in Western blot, we have found that ab113691 typically gives a more robust signal. Therefore, if you plan on using this antibody in WB only, we recommend ab113691 as an alternative.



Our Abpromise guarantee covers the use of ab110328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 3 - 5 µg/ml. Predicted molecular weight: 23 kDa. (A chemiluminescent detection system is highly recommended)
ICC/IF Use a concentration of 0.5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-P Use a concentration of 10 µg/ml.


  • Function
    Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+); the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. Modulates the RNA-binding activity of ACO1.
  • Tissue specificity
    Expressed in the heart, peripheral blood lymphocytes and dermal fibroblasts.
  • Involvement in disease
    Defects in FXN are the cause of Friedreich ataxia (FRDA) [MIM:229300]. FRDA is an autosomal recessive, progressive degenerative disease characterized by neurodegeneration and cardiomyopathy it is the most common inherited ataxia. The disorder is usually manifest before adolescence and is generally characterized by incoordination of limb movements, dysarthria, nystagmus, diminished or absent tendon reflexes, Babinski sign, impairment of position and vibratory senses, scoliosis, pes cavus, and hammer toe. In most patients, FRDA is due to GAA triplet repeat expansions in the first intron of the frataxin gene. But in some cases the disease is due to mutations in the coding region.
  • Sequence similarities
    Belongs to the frataxin family.
  • Post-translational
    Processed in two steps by mitochondrial processing peptidase (MPP). MPP first cleaves the precursor to intermediate form and subsequently converts the intermediate to yield frataxin mature form (frataxin(81-210)) which is the predominant form. The additional forms, frataxin(56-210) and frataxin(78-210), seem to be produced when the normal maturation process is impaired; their physiological relevance is unsure.
  • Cellular localization
    Cytoplasm. Mitochondrion. PubMed:18725397 reports localization exclusively in mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • CyaY antibody
    • d-FXN antibody
    • FA antibody
    • FARR antibody
    • Frataxin mature form antibody
    • Frataxin(81-210) antibody
    • FRDA antibody
    • FRDA_HUMAN antibody
    • Friedreich ataxia protein antibody
    • Fxn antibody
    • i-FXN antibody
    • m56-FXN antibody
    • m78-FXN antibody
    • m81-FXN antibody
    • MGC57199 antibody
    • X25 antibody
    see all


  • IHC image of Frataxin staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110328, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry analysis using ab110328 at 0.5µg/ml staining Frataxin in normal Human fibroblasts (MRC5) (fixed and permeabilized) followed by an AlexaFluor® 594-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml). .
  • Flow cytometric analysis using ab110328 at 1µg/ml staining Frataxin in HL60 cells (blue). Isotype control antibody (red).
  • Anti-Frataxin antibody [18A5DB1] (ab110328) at 5 µg/ml + Recombinant Human Frataxin at 0.0005 µg

    Predicted band size: 23 kDa


This product has been referenced in:
  • Distante S  et al. Blood removal therapy in hereditary hemochromatosis induces a stress response resulting in improved genome integrity. Transfusion 56:1435-41 (2016). Read more (PubMed: 27045387) »
  • Chapdelaine P  et al. Development of an AAV9 coding for a 3XFLAG-TALEfrat#8-VP64 able to increase in vivo the human frataxin in YG8R mice. Gene Ther 23:606-14 (2016). WB ; Human . Read more (PubMed: 27082765) »

See all 19 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (13 % separating gel 5 % collecting gel)
Human Cell lysate - other (see additional notes)
see additional notes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 22 2014

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Human Cell (SH-SY5Y human neuroblastoma)
SH-SY5Y human neuroblastoma
Yes - 0.2% NP-40, 3min

Dr. George Allen

Verified customer

Submitted Dec 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (fibroblasts)
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 12 2013

Thank you for your email, I am sorry for the delay in getting back to you.

I have a couple of antibodies your customer may be interested in:

Anti-Frataxin antibody [18A5DB1] (ab110328)


Anti-ATP5J antibody [10C6AC...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab48281.
To check the status of the order please contact our Cus...

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Thank you for contacting us. I apologize for the waiting.
Both antibodies were raised against the mature protein: Recombinant full length Human Frataxin (amino acids 56-210). Unfortunately we have not done epitope mapping and therefore we are unab...

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Following my previous email, I would like to inform you that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement or a c...

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Thank you for contacting us.
Please find attached to this email the recommended flow cytometry protocol. Also below I copied a brief protocol for staining with the anti-Frataxin antibody [18A5DB1] (ref. ab110328). As isotype control I would recomm...

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Thank you for contacting Abcam.

Please find attached the protocol that was used to test ab110328 in flow cytometry.

If there is anything else I can help you with, please let me know.

1-10 of 13 Abreviews or Q&A


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