Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768)

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Overview

  • Product name

    Free Fatty Acid Uptake Assay Kit (Fluorometric)

  • Detection method

    Fluorescent

  • Sample type

    Adherent cells

  • Assay type

    Cell-based (quantitative)

  • Assay time

    1h 00m

  • Species reactivity

    Reacts with: Mammals, Other species

  • Product overview

    Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768) provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters. The kit uses a proprietary dodecanoic acid fluorescent fatty acid substrate. This fatty acid uptake assay kit can be performed on any fluorescence microplate reader with a bottom-read mode at Ex/Em = 485/515 nm or FITC channel. The assay can be performed in 96-well or 384-well microtiter plates in a simple mix-and-read procedure, and easily adapted for high throughput screening applications.

  • Notes

    Fatty acid uptake is an important therapeutic target for the treatment of many human diseases such as obesity, type 2 diabetes and hepatic steatosis.

  • Platform

    Microplate reader

Properties

Images

  • Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.
    Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.

    Cells were plated at 50,000 cells/100μL/well in a 96 well black wall/clear bottom poly-D lysine plate for 5 hours, and then serum deprived for 1 hour. Cells were treated without (control) or with insulin (150 nM), and incubated at 37 ºC, 5% CO2 incubator for 30 min. At the end of the incubation time, 100 μL of fatty acid mixture was added into the well, and incubated for another 60 min, the fluorescence signal was measured with a plate reader using bottom read mode. A – fibroblasts (Control); B – fibroblasts (Insulin); C – adipocytes (Control); D– adipocytes (Insulin).

  • Excitation and Emission Spectra of Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768)
    Excitation and Emission Spectra of Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768)

Protocols

References

This product has been referenced in:

  • Yen MC  et al. New Insight on Solute Carrier Family 27 Member 6 (SLC27A6) in Tumoral and Non-Tumoral Breast Cells. Int J Med Sci 16:366-375 (2019). Read more (PubMed: 30911270) »
  • Sun Y  et al. miR-142 controls metabolic reprogramming that regulates dendritic cell activation. J Clin Invest 129:2029-2042 (2019). Read more (PubMed: 30958798) »
See all 10 Publications for this product

Publishing research using ab176768? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Negative uptake

 Poor Good 4/5 (Ease of Use)
Abreviews
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We tried to measure fatty acid uptake in cardiomyocytes using ab176768, but our attempts were unsuccessful for 2 reasons. 1) In some experiments, "uptake" was negative, ie the fluorescence reading decreased over time. 2) In other experiments, the reading in my cells were lower than cell-free blank readings. This occurred despite many attempts to optimise using different time points, different cell types (cardiomyocytes, adipocytes), and different plating densities. Technical support was contacted but unfortunately testing was still unsuccessful

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Submitted Mar 05 2018

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