Free Glycerol Assay Kit (Fluorometric, High Sensitivity) (ab174092)

Overview

  • Product name
    Free Glycerol Assay Kit (Fluorometric, High Sensitivity)
    See all Glycerol kits
  • Detection method
    Fluorescent
  • Sample type
    Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    0.04 nmol/well
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    Abcam's PicoProbe Free Glycerol Assay Kit (ab174092) is simple, sensitive and easy to use tool to detect small amounts of glycerol.


    This assay is suitable for measuring trace amount of glycerol in samples containing reducing substances, which may interfere with oxidase-based assays. In the assay, glycerol reacts with the enzyme mix to form an intermediate, which is subsequently oxidized with the production of fluorescence. The fluorescence intensity is directly proportional to the amount of glycerol.


    This assay kit can detect glycerol amount less than 40 pmol.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glycerol is a central component for synthesis of all lipids; it acts as a backbone for triglycerides and phospholipids, which plays an important role for cell membrane’s structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic industries.

  • Platform
    Microplate

Properties

Images

  • Free Glycerol measured in cell lysates showing quantity (pmol) per 1 mln cells.

    Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 2-6 fold.

  • Example of Glycerol standard curve following assay's protocol.

  • Example of Glycerol standard curve following assay's protocol.

  • Measurement of glycerol levels in rat heart (5 µg protein), rat kidney (5 µg) and Jurkat cell lysate (10 µg) sample following PicoProbe Free Glycerol Assay Kit (ab174092) protocol. This is example data only.

Protocols

References

ab174092 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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