Overview

  • Product name
    Free Glycerol Assay Kit II (Colorimetric)
    See all Glycerol kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
  • Assay type
    Quantitative
  • Sensitivity
    < 20 µM
  • Range
    2 nmol/well - 10 nmol/well
  • Assay time
    1h 30m
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Abcam’s Free Glycerol Assay kit II (ab155899) is suitable for measuring free glycerol levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, Glycerol in the presence of Glycerol Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. Free Glycerol Assay Kit II is simple, rapid and high-throughput adaptable. This assay kit can detect less than 20 μM of free Glycerol in various biological samples.


    Detection method: colorimetric – based on enzyme activity

  • Notes

    Glycerol is a central component of most lipids. It acts as a backbone for triglycerides and phospholipids, which play an important role in metabolism and cell membrane structure. Due to its low toxicity, glycerol is widely used in the pharmaceutical, food and cosmetic industries.

Properties

Images

  • Glycerol Standard Curve [a]. Measurement of free glycerol in bovine liver (50 µg), Jurkat cells (40 µg), HeLa cells (30 µg), fish egg (58 µg) & human serum (2 µl) [b]. Assays were performed following kit protocol.

Protocols

References

ab155899 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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