Product nameFree Glycerol Assay Kit II (Colorimetric)
See all Glycerol kits
Sample typeCell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Sensitivity< 20 µM
Range2 nmol/well - 10 nmol/well
Assay time1h 30m
Species reactivityReacts with: Mammals, Other species
Abcam’s Free Glycerol Assay kit II (ab155899) is suitable for measuring free glycerol levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, Glycerol in the presence of Glycerol Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. Free Glycerol Assay Kit II is simple, rapid and high-throughput adaptable. This assay kit can detect less than 20 μM of free Glycerol in various biological samples.
Detection method: colorimetric – based on enzyme activity
Glycerol is a central component of most lipids. It acts as a backbone for triglycerides and phospholipids, which play an important role in metabolism and cell membrane structure. Due to its low toxicity, glycerol is widely used in the pharmaceutical, food and cosmetic industries.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Glycerol Assay Buffer WM 1 x 25ml Glycerol Enzyme Mix (lyophilized) Green 1 vial Glycerol Probe Red 1 vial Glycerol Standard Yellow 1 x 0.2ml
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
RelevanceGlycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development.
ab155899 has not yet been referenced specifically in any publications.