• Product name
  • Description
    Rabbit polyclonal to Frizzled 5
  • Host species
  • Specificity
    Recognizes human Fz5.
  • Tested applications
    Suitable for: ICC/IF, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to N terminal amino acids 564-585 of Human Frizzled 5.



Our Abpromise guarantee covers the use of ab14475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20530549
IHC-FoFr Use at an assay dependent concentration. PubMed: 20530549
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.


  • Function
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain
    The PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization
    Cell membrane. Golgi apparatus membrane. Localized at the plasma membrane and also found at the Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2orf31 antibody
    • DKFZP434E2135 antibody
    • Frizzled 5 seven transmembrane spanning receptor antibody
    • Frizzled class receptor 5 antibody
    • Frizzled family receptor 5 antibody
    • Frizzled homolog 5 antibody
    • Frizzled-5 antibody
    • Frizzled5 antibody
    • Fz 5 antibody
    • Fz-5 antibody
    • Fz5 antibody
    • FZD 5 antibody
    • fzd5 antibody
    • FZD5_HUMAN antibody
    • FzE 5 antibody
    • FzE5 antibody
    • HFZ 5 antibody
    • hFz5 antibody
    • MGC129692 antibody
    • Seven transmembrane receptor frizzled 5 antibody
    • Uncharacterized protein C2orf31 antibody
    • Wnt receptor antibody
    see all


  • TENT lysates of transiently transfected 293 cells were probed with anti-Fz5 (1 g/ml). TENT lysing buffer; 20mM Tris-HCl, pH 8.0, 2mM EDTA, 150mM NaCl, and 1% Triton X-100; 1mM PMSF; 1ug/ml each aprotinin, leupeptin, pepstatin; 1mM Na3VO4; 1mM NaF


This product has been referenced in:

See all 4 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Prostate cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Prostate cancer
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 13 2015

Thank you for contacting us.

Your credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed agains...

Read More

Thank you very much for keeping me updated about the results, and I am so glad to hear that the new lot is working better than the previous one.

We currently only have this newer lot in stock. If you'd like, you can contact me before placing ...

Read More

Thank you very much for confirming these details.

I'm sending two free of charge vials of ab14475 from a new lot on the order *** which should arrive tomorrow.

Please keep me updated about the results with these replacement vials, a...

Read More

Thank you very much for your reply and for sending the additional information.

I am sorry for all of the frustration and inconvenience regarding the poor performance of this antibody. I will be happy to send a new lot. Could you please confir...

Read More

Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab regarding this antibody.

The predicted molecular weight is based only on the precursor amino acid sequence of Frizzled 5,...

Read More

Thank you for your enquiry. We currently have three antibodies against Frizzled 5. Further to clarification with the lab, only one of them has been tested for use in western blot; Frizzled 5 antibody (ab14475). I have uploaded an image of this immu...

Read More


Sign up