Overview

  • Product name
  • Description
    Rabbit polyclonal to Frizzled 5
  • Host species
    Rabbit
  • Specificity
    Recognizes human Fz5.
  • Tested applications
    Suitable for: ICC/IF, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    C-TGRTPPGPAATYHKQVSLSHV

    conjugated to KLH, corresponding to N terminal amino acids 564-585 of Human Frizzled 5.

Properties

Applications

Our Abpromise guarantee covers the use of ab14475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20530549
IHC-FoFr Use at an assay dependent concentration. PubMed: 20530549
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.

Target

  • Function
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain
    The PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization
    Cell membrane. Golgi apparatus membrane. Localized at the plasma membrane and also found at the Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2orf31 antibody
    • DKFZP434E2135 antibody
    • Frizzled 5 seven transmembrane spanning receptor antibody
    • Frizzled class receptor 5 antibody
    • Frizzled family receptor 5 antibody
    • Frizzled homolog 5 antibody
    • Frizzled-5 antibody
    • Frizzled5 antibody
    • Fz 5 antibody
    • Fz-5 antibody
    • Fz5 antibody
    • FZD 5 antibody
    • fzd5 antibody
    • FZD5_HUMAN antibody
    • FzE 5 antibody
    • FzE5 antibody
    • HFZ 5 antibody
    • hFz5 antibody
    • MGC129692 antibody
    • Seven transmembrane receptor frizzled 5 antibody
    • Uncharacterized protein C2orf31 antibody
    • Wnt receptor antibody
    see all

Images

  • TENT lysates of transiently transfected 293 cells were probed with anti-Fz5 (1 g/ml). TENT lysing buffer; 20mM Tris-HCl, pH 8.0, 2mM EDTA, 150mM NaCl, and 1% Triton X-100; 1mM PMSF; 1ug/ml each aprotinin, leupeptin, pepstatin; 1mM Na3VO4; 1mM NaF

References

This product has been referenced in:
See all 4 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
Prostate cancer
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 13 2015

Answer

Thank you for contacting us.

Your credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you very much for keeping me updated about the results, and I am so glad to hear that the new lot is working better than the previous one.

We currently only have this newer lot in stock. If you'd like, you can contact me before placing another order and I can check our stock to make sure this is still the case. You can also request a specific lot number anytime you place an order with us.

Please let me know if there is anything else that I can do for you or if you have any questions. Have a great day.

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Question
Answer

Thank you very much for confirming these details.

I'm sending two free of charge vials of ab14475 from a new lot on the order *** which should arrive tomorrow.

Please keep me updated about the results with these replacement vials, and let me know if there is anything else that we can do for you. Have a nice day.

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Answer

Thank you very much for your reply and for sending the additional information.

I am sorry for all of the frustration and inconvenience regarding the poor performance of this antibody. I will be happy to send a new lot. Could you please confirm your order number, PO number, or mailing address so that I can send the replacement?

I look forward to hearing from you. Please let me know if there is anything else that we can do for you, and I'll be happy to help.

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Answer

Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab regarding this antibody.

The predicted molecular weight is based only on the precursor amino acid sequence of Frizzled 5, and after the signal peptide is cleaved the protein will have a lower molecular weight. The lower band below 62 kDa corresponds to the cleaved mature protein. The lab scientists have not further characterized the higher band above 200 kDa, and ithas been hypothesized that this isan aggregate of the protein, but I haven't found support for this in the literature. The image shows a transfected lysate, so perhaps it is a product of the transfection, but it may also be a non-specific band. Could you tell me what kind of lysates you are using? How are the samples prepared? What blocking solution is used, and what dilution of antibody has been tried? What is the lot number associated to each of these vials that you've tried?

I look forward to hearing from you. We do fully guarantee this antibody to work in the tested species and applications listed on the datasheet for up to 6 months after purchase, so if the results are not satisfactory we may be able to send a replacement or issue a credit or refund.

Please let me know if you have any further questions or if there is anything else that we can do for you, and I'll be happy to help.

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Answer

Thank you for your enquiry. We currently have three antibodies against Frizzled 5. Further to clarification with the lab, only one of them has been tested for use in western blot; Frizzled 5 antibody (ab14475). I have uploaded an image of this immunoblot to the datasheet. You can examine this by clicking on the link below. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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