Key features and details
- Rabbit polyclonal to Frizzled 6 - N-terminal
- Suitable for: IHC-P, ICC
- Reacts with: Human, Non human primates
- Isotype: IgG
Product nameAnti-Frizzled 6 antibody - N-terminal
See all Frizzled 6 primary antibodies
DescriptionRabbit polyclonal to Frizzled 6 - N-terminal
Tested applicationsSuitable for: IHC-P, ICCmore details
Species reactivityReacts with: Human, Non human primates
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig
Synthetic peptide, corresponding to 16 amino acid residues from the N terminal, extracellular domain of Human Frizzled 6 (NP_003497.2).
- Human pancreas tissue; HEK293 cells.
Storage instructionsShipped at 4°C.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 6 - 14 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||Use a concentration of 3 µg/ml.|
FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear.
Tissue specificityDetected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney.
Involvement in diseaseNail disorder, non-syndromic congenital, 10
Rare non-synonymous variants in FZD6 may contribute to neural tube defects, congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy.
Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands.
modificationsUbiquitinated by ZNRF3, leading to its degradation by the proteasome.
Cellular localizationMembrane. Cell membrane. Cell surface. Apical cell membrane. Cytoplasmic vesicle membrane. Colocalizes with FZD3 at the apical face of cells (By similarity).
- Information by UniProt
- Frizzled 6 seven transmembrane spanning receptor antibody
- Frizzled drosophila homolog of 6 antibody
- Frizzled family receptor 6 antibody
ab150545 has been referenced in 2 publications.
- Xu C et al. NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/ß-catenin pathway via FZD6. Cell Death Dis 10:217 (2019). PubMed: 30833544
- Bhattarai SR et al. The ciliary GTPase Arl3 maintains tissue architecture by directing planar spindle orientation during epidermal morphogenesis. Development 146:N/A (2019). PubMed: 30952667