Overview

  • Product name

    Anti-Frizzled 6 antibody - N-terminal
    See all Frizzled 6 primary antibodies
  • Description

    Rabbit polyclonal to Frizzled 6 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide within Human Frizzled 6 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated
    Database link: O60353

  • Positive control

    • A431, HeLa, HepG2, Jurkat, Raji, NCI-H929 and unboiled HEK-293T cell extracts; Boiled HEK-293T membrane extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 79 kDa.

Target

  • Function

    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear.
  • Tissue specificity

    Detected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney.
  • Involvement in disease

    Nail disorder, non-syndromic congenital, 10
    Rare non-synonymous variants in FZD6 may contribute to neural tube defects, congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy.
  • Sequence similarities

    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain

    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Post-translational
    modifications

    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • Cellular localization

    Membrane. Cell membrane. Cell surface. Apical cell membrane. Cytoplasmic vesicle membrane. Colocalizes with FZD3 at the apical face of cells (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • Frizzled 6 seven transmembrane spanning receptor antibody
    • Frizzled drosophila homolog of 6 antibody
    • Frizzled family receptor 6 antibody
    • Frizzled homolog 6 (Drosophila) antibody
    • Frizzled homolog 6 antibody
    • Frizzled-6 antibody
    • Frizzled6 antibody
    • Fz 6 antibody
    • Fz-6 antibody
    • Fz6 antibody
    • FZD 6 antibody
    • Fzd6 antibody
    • FZD6_HUMAN antibody
    • Hfz 6 antibody
    • hFz6 antibody
    • NDNC10 antibody
    see all

Images

  • All lanes : Anti-Frizzled 6 antibody - N-terminal (ab229663) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 5 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 6 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract
    Lane 7 : Unboiled HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 8 : Unboiled HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) membrane extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 79 kDa



    7.5% SDS-PAGE gel.

References

ab229663 has not yet been referenced specifically in any publications.

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