Product nameAnti-Frizzled 6 antibody - N-terminal
See all Frizzled 6 primary antibodies
DescriptionRabbit polyclonal to Frizzled 6 - N-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Synthetic peptide within Human Frizzled 6 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated
Database link: O60353
- A431, HeLa, HepG2, Jurkat, Raji, NCI-H929 and unboiled HEK-293T cell extracts; Boiled HEK-293T membrane extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab229663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 79 kDa.|
FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear.
Tissue specificityDetected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney.
Involvement in diseaseNail disorder, non-syndromic congenital, 10
Rare non-synonymous variants in FZD6 may contribute to neural tube defects, congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy.
Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands.
modificationsUbiquitinated by ZNRF3, leading to its degradation by the proteasome.
Cellular localizationMembrane. Cell membrane. Cell surface. Apical cell membrane. Cytoplasmic vesicle membrane. Colocalizes with FZD3 at the apical face of cells (By similarity).
- Information by UniProt
- Frizzled 6 seven transmembrane spanning receptor antibody
- Frizzled drosophila homolog of 6 antibody
- Frizzled family receptor 6 antibody
All lanes : Anti-Frizzled 6 antibody - N-terminal (ab229663) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 5 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 6 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract
Lane 7 : Unboiled HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 8 : Unboiled HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) membrane extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 79 kDa
7.5% SDS-PAGE gel.
ab229663 has not yet been referenced specifically in any publications.