Product nameAnti-Frizzled 7 antibody
See all Frizzled 7 primary antibodies
DescriptionRabbit polyclonal to Frizzled 7
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Dog
- HepG2 cell lysate, Human kidney This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab51049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Tissue specificityHigh expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
DomainLys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands.
- Information by UniProt
- Frizzled 7, seven transmembrane spanning receptor antibody
- frizzled class receptor 7 antibody
- Frizzled drosophila homolog of 7 antibody
Lane 1 : Marker
Lane 2 : Anti-Frizzled 7 antibody (ab51049) at 1 µg/ml
Lane 2 : HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Gel concentration: 12%
ICC/IF image of ab51049 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab51049 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab51049 at 4.0 µg/ml staining epithelial cells of renal tubule from human kidney; paraffin embedded; magnification 400x.
Lane 2 : Anti-Frizzled 7 antibody (ab51049) at 2 µg/ml
Lane 3 : Anti-Frizzled 7 antibody (ab51049) at 1 µg/ml
Lane 4 : Anti-Frizzled 7 antibody (ab51049) at 0.5 µg/ml
Lane 5 : Anti-Frizzled 7 antibody (ab51049) at 0.25 µg/ml
All lanes : Cell lysate prepared from HepG2 cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 64 kDa
This product has been referenced in:
- Yamaguchi S et al. Wnt-2b in the intermediate hyperpallium apicale of the telencephalon is critical for the thyroid hormone-mediated opening of the sensitive period for filial imprinting in domestic chicks (Gallus gallus domesticus). Horm Behav 102:120-128 (2018). IHC-Fr ; Chicken . Read more (PubMed: 29778460) »
- Lo RC et al. Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/ß-catenin pathway. Cell Death Differ 25:1426-1441 (2018). Read more (PubMed: 29445127) »