Overview

  • Product name

    Anti-Frizzled 7 antibody - C-terminal
    See all Frizzled 7 primary antibodies
  • Description

    Goat polyclonal to Frizzled 7 - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Chicken, Hamster, Cow, Dog, Xenopus laevis, Monkey, Opossum
  • Immunogen

    Synthetic peptide corresponding to Human Frizzled 7 aa 559-572 (C terminal).
    Sequence:

    RFYHRLSHSSKGET


    Database link: O75084

  • Positive control

    • HepG2 cell lysate, Human spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab188633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 64 kDa.

Target

  • Function

    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificity

    High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
  • Sequence similarities

    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain

    Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Frizzled 7, seven transmembrane spanning receptor antibody
    • frizzled class receptor 7 antibody
    • Frizzled drosophila homolog of 7 antibody
    • Frizzled family receptor 7 antibody
    • Frizzled homolog 7 antibody
    • Frizzled-7 antibody
    • Frizzled7 antibody
    • Fz 7 antibody
    • Fz-7 antibody
    • Fz7 antibody
    • FZD 7 antibody
    • FZD7 antibody
    • FZD7_HUMAN antibody
    • FzE 3 antibody
    • FzE3 antibody
    • hFz 7 antibody
    • hFz7 antibody
    • Homolog of Drosophila Frizzled 7 antibody
    • OTTHUMP00000163742 antibody
    see all

Images

  • Anti-Frizzled 7 antibody - C-terminal (ab188633) at 0.3 µg/ml + HepG2 cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 64 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue, labeling Frizzled 7 using ab188633 at 5 µg/ml.

References

ab188633 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab188633.
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