Overview

  • Product name
    Anti-Frizzled 8 antibody [EPR7308(2)]
    See all Frizzled 8 primary antibodies
  • Description
    Rabbit monoclonal [EPR7308(2)] to Frizzled 8
  • Host species
    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the 100% homology with the immunogen. We do not have experimental data in mouse and rat species.

  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Frizzled 8 aa 650-750. The exact sequence is proprietary.

  • Epitope
    HeLa and Jurkat lysates; Human endometrial carcinoma tissue.
  • Positive control
    • WB: Jurkat whole cell lysate (ab7899), HeLa, 293T, PC-3, Human breast cancer lysates, Human prostate cancer lysates, Human prostate lysates, Human lung cancer lysates, Human lung lysates IHC-P: Human breast carcinoma
  • General notes

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovations Ltd. Rhodadyn is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
    • Tissue specificity
      Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
    • Sequence similarities
      Belongs to the G-protein coupled receptor Fz/Smo family.
      Contains 1 FZ (frizzled) domain.
    • Domain
      The PDZ-binding motif mediates interaction with GOPC.
      Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
      The FZ domain is involved in binding with Wnt ligands.
    • Post-translational
      modifications
      Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
    • Cellular localization
      Membrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Frizzled 8 seven transmembrane spanning receptor antibody
      • frizzled 8, seven transmembrane spanning receptor antibody
      • Frizzled family receptor 8 antibody
      • frizzled homolog 8 (Drosophila) antibody
      • Frizzled homolog 8 antibody
      • Frizzled-8 antibody
      • FZ 8 antibody
      • Fz-8 antibody
      • FZ8 antibody
      • FZD 8 antibody
      • FZD8 antibody
      • FZD8_HUMAN antibody
      • hFZ 8 antibody
      • hFz8 antibody
      • HGNC4046 antibody
      • Homolog of Drosophila Frizzled 8 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Frizzled 8 with Purified ab155093 at 1:500 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • All lanes : Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093) at 1/1000 dilution

      Lane 1 : Human prostate cancer lysates
      Lane 2 : Human prostate lysates
      Lane 3 : Human lung cancer lysates
      Lane 4 : Human lung lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 73 kDa



      The expression level of Frizzled 8 in normal tissues is low and can be upregulated in cancer tissues reported by PMID 23815780 and 22138402

    • All lanes : Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 3 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
      Lane 4 : Human breast cancer lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 73 kDa
      Observed band size: 73 kDa

    • All lanes : Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 73 kDa



      Primary antibody diluted with 1% BSA.

    • Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling Frizzled 8 with ab155093 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab155093 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155093 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155093 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab155093 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded human normal pancreas tissue using ab155093 showing +ve staining.

    References

    ab155093 has not yet been referenced specifically in any publications.

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