Recombinant
RabMAb

Recombinant Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)

Overview

  • Product name

    Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free
    See all Frizzled 8 primary antibodies
  • Description

    Rabbit monoclonal [EPR7308(2)] to Frizzled 8 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the 100% homology with the immunogen. We do not have experimental data in mouse and rat species. 

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Frizzled 8 aa 650-750. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human normal pancreas tissue and Human breast carcinoma.
  • General notes

    Ab238967 is the carrier-free version of ab155093. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238967 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the 100% homology with the immunogen. We do not have experimental data in mouse and rat species. 

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Predicted molecular weight: 73 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
    • Tissue specificity

      Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
    • Sequence similarities

      Belongs to the G-protein coupled receptor Fz/Smo family.
      Contains 1 FZ (frizzled) domain.
    • Domain

      The PDZ-binding motif mediates interaction with GOPC.
      Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
      The FZ domain is involved in binding with Wnt ligands.
    • Post-translational
      modifications

      Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
    • Cellular localization

      Membrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Frizzled 8 seven transmembrane spanning receptor antibody
      • frizzled 8, seven transmembrane spanning receptor antibody
      • Frizzled family receptor 8 antibody
      • frizzled homolog 8 (Drosophila) antibody
      • Frizzled homolog 8 antibody
      • Frizzled-8 antibody
      • FZ 8 antibody
      • Fz-8 antibody
      • FZ8 antibody
      • FZD 8 antibody
      • FZD8 antibody
      • FZD8_HUMAN antibody
      • hFZ 8 antibody
      • hFz8 antibody
      • HGNC4046 antibody
      • Homolog of Drosophila Frizzled 8 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Frizzled 8 with Purified ab155093 at 1:500 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093)
    • Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling Frizzled 8 with ab155093 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab155093 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155093 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155093 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab155093 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded human normal pancreas tissue using ab155093 at a 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, tissue culture supernatant and sodium azide (ab155093).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab238967 has not yet been referenced specifically in any publications.

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