• Product name

    Anti-Frizzled 8 antibody - Extracellular domain
    See all Frizzled 8 primary antibodies
  • Description

    Rabbit polyclonal to Frizzled 8 - Extracellular domain
  • Host species

  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except INTS6 (50%).
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Lizard, Opossum, Common marmoset, Elephant
  • Immunogen

    Synthetic 16 amino acid peptide from the third extracellular domain of Human Frizzled 8.

  • Positive control

    • Human brain cerebellum purkinje neurons and Human fetal kidney tissue



Our Abpromise guarantee covers the use of ab150500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 8 - 20 µg/ml.


  • Function

    Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
  • Tissue specificity

    Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
  • Sequence similarities

    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain

    The PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Post-translational

    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • Cellular localization

    Membrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Frizzled 8 seven transmembrane spanning receptor antibody
    • frizzled 8, seven transmembrane spanning receptor antibody
    • Frizzled family receptor 8 antibody
    • frizzled homolog 8 (Drosophila) antibody
    • Frizzled homolog 8 antibody
    • Frizzled-8 antibody
    • FZ 8 antibody
    • Fz-8 antibody
    • FZ8 antibody
    • FZD 8 antibody
    • FZD8 antibody
    • FZD8_HUMAN antibody
    • hFZ 8 antibody
    • hFz8 antibody
    • HGNC4046 antibody
    • Homolog of Drosophila Frizzled 8 antibody
    see all


  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cerebellum purkinje neurons labelling Frizzled 8 with ab150500 at 20 µg/ml.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal kidney tissue labelling Frizzled 8 with ab150500 at 20 µg/ml.


ab150500 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Small intestine, Jejunum, Paneth cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Yes - 3 % H2O2
Small intestine, Jejunum, Paneth cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

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Submitted Apr 03 2017

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