Anti-Frizzled 8 antibody - Extracellular domain (ab150500)
Key features and details
- Rabbit polyclonal to Frizzled 8 - Extracellular domain
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Frizzled 8 antibody - Extracellular domain
See all Frizzled 8 primary antibodies -
Description
Rabbit polyclonal to Frizzled 8 - Extracellular domain -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except INTS6 (50%). -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Lizard, Opossum, Common marmoset, Elephant -
Immunogen
Synthetic 16 amino acid peptide from the third extracellular domain of Human Frizzled 8.
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Positive control
- Human brain cerebellum purkinje neurons and Human fetal kidney tissue
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 8 - 20 µg/ml.
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Notes |
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IHC-P
Use a concentration of 8 - 20 µg/ml. |
Target
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Function
Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1. -
Tissue specificity
Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle. -
Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
Domain
The PDZ-binding motif mediates interaction with GOPC.
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
Post-translational
modificationsUbiquitinated by ZNRF3, leading to its degradation by the proteasome. -
Cellular localization
Membrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 100398598 Common marmoset
- Entrez Gene: 616913 Cow
- Entrez Gene: 8325 Human
- Entrez Gene: 14370 Mouse
- Entrez Gene: 100909849 Rat
- Entrez Gene: 364754 Rat
- Omim: 606146 Human
- SwissProt: Q9H461 Human
see all -
Alternative names
- Frizzled 8 seven transmembrane spanning receptor antibody
- frizzled 8, seven transmembrane spanning receptor antibody
- Frizzled family receptor 8 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab150500 has been referenced in 1 publication.
- Ma P & Han J Overexpression of miR-100-5p inhibits papillary thyroid cancer progression via targeting FZD8. Open Med (Wars) 17:1172-1182 (2022). PubMed: 35859793