Overview

  • Product name

    Anti-Frizzled 8 antibody - Extracellular domain
    See all Frizzled 8 primary antibodies
  • Description

    Rabbit polyclonal to Frizzled 8 - Extracellular domain
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except INTS6 (50%).
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Lizard, Opossum, Common marmoset, Elephant
  • Immunogen

    Synthetic 16 amino acid peptide from the third extracellular domain of Human Frizzled 8.

  • Positive control

    • Human brain cerebellum purkinje neurons and Human fetal kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab150500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 8 - 20 µg/ml.

Target

  • Function

    Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
  • Tissue specificity

    Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
  • Sequence similarities

    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain

    The PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Post-translational
    modifications

    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • Cellular localization

    Membrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Frizzled 8 seven transmembrane spanning receptor antibody
    • frizzled 8, seven transmembrane spanning receptor antibody
    • Frizzled family receptor 8 antibody
    • frizzled homolog 8 (Drosophila) antibody
    • Frizzled homolog 8 antibody
    • Frizzled-8 antibody
    • FZ 8 antibody
    • Fz-8 antibody
    • FZ8 antibody
    • FZD 8 antibody
    • FZD8 antibody
    • FZD8_HUMAN antibody
    • hFZ 8 antibody
    • hFz8 antibody
    • HGNC4046 antibody
    • Homolog of Drosophila Frizzled 8 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cerebellum purkinje neurons labelling Frizzled 8 with ab150500 at 20 µg/ml.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal kidney tissue labelling Frizzled 8 with ab150500 at 20 µg/ml.

References

ab150500 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Small intestine, Jejunum, Paneth cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Permeabilization
Yes - 3 % H2O2
Specification
Small intestine, Jejunum, Paneth cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

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Verified customer

Submitted Apr 03 2017

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