Overview

  • Product name
  • Description
    Rabbit polyclonal to Frizzled 9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Frizzled 9 aa 562-591 (C terminal). The exact sequence is proprietary.
    Database link: O00144

  • Positive control
    • HUVEC cell lysate; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab195718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 64 kDa.
ICC/IF 1/100.

Target

  • Function
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificity
    Expressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. Moderately expressed in pancreas, thyroid, adrenal cortex, small intestine and stomach. Detected in fetal liver and kidney.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 349 antibody
    • CD 349 antigen antibody
    • CD349 antibody
    • Frizzled Drosophila homolog of 3 antibody
    • Frizzled homolog 9 (Drosophila) antibody
    • Frizzled homolog 9 antibody
    • Frizzled-9 antibody
    • Fz 9 antibody
    • Fz-9 antibody
    • FZD 3 antibody
    • Fzd 9 antibody
    • FZD3 antibody
    • FZD3-PEN antibody
    • FZD9 antibody
    • FZD9_HUMAN antibody
    • FzE6 antibody
    • hFz9 antibody
    • mfz9 antibody
    • Xfz9 antibody
    see all

Images

  • Immunofluorescent analysis of A549 cells labeling Frizzled 9 with ab195718 at 1/100 dilution. The image in the right is the negative control using PBS only.

  • Anti-Frizzled 9 antibody (ab195718) at 1/1000 dilution + HUVEC cell lysate at 35 µg

    Secondary
    goat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

    Predicted band size: 64 kDa

References

This product has been referenced in:
  • Chen X  et al. The hedgehog and Wnt/ß-catenin system machinery mediate myofibroblast differentiation of LR-MSCs in pulmonary fibrogenesis. Cell Death Dis 9:639 (2018). Read more (PubMed: 29844390) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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