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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-Frizzled homolog 1 antibody (ab117305)

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Western blot - Anti-Frizzled homolog 1 antibody (ab117305)

    Key features and details

    • Rabbit polyclonal to Frizzled homolog 1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Frizzled homolog 1 protein (Fc Chimera) (ab198659)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Frizzled homolog 1 antibody
      See all Frizzled homolog 1 primary antibodies
    • Description

      Rabbit polyclonal to Frizzled homolog 1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
    • Immunogen

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • This antibody gave a positive signal in the following Human tissue lysates: Heart; Placenta; Lung; Kidney; Pancreas.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR
      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Surface Molecules
      • Cancer
      • Signal transduction
      • G protein signaling
      • GPCR

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Frizzled homolog 1 protein (Fc Chimera) (ab198659)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab117305 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).

    Target

    • Function

      Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B.
    • Tissue specificity

      Expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney.
    • Sequence similarities

      Belongs to the G-protein coupled receptor Fz/Smo family.
      Contains 1 FZ (frizzled) domain.
    • Domain

      Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
      The FZ domain is involved in binding with Wnt ligands.
    • Cellular localization

      Membrane. Cell membrane.
    • Target information above from: UniProt accession Q9UP38 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 445417 Cow
      • Entrez Gene: 482294 Dog
      • Entrez Gene: 101126536 Gorilla
      • Entrez Gene: 100060569 Horse
      • Entrez Gene: 8321 Human
      • Entrez Gene: 14362 Mouse
      • Entrez Gene: 58868 Rat
      • Omim: 603408 Human
      • SwissProt: Q9UP38 Human
      • SwissProt: O70421 Mouse
      • SwissProt: Q08463 Rat
      • Unigene: 94234 Human
      • Unigene: 246003 Mouse
      • Unigene: 6575 Rat
      see all
    • Alternative names

      • Chfz-1 antibody
      • Dfrizzled-1 antibody
      • DKFZp564G072 antibody
      • FLJ95923 antibody
      • Frizzled 1 antibody
      • Frizzled homolog 1 (Drosophila) antibody
      • Frizzled homolog 1 antibody
      • Frizzled-1 antibody
      • Fz receptor 1 antibody
      • Fz-1 antibody
      • Fz1 antibody
      • FZD1 antibody
      • FZD1_HUMAN antibody
      • FzE1 antibody
      • hFz1 antibody
      • Rfz1 antibody
      • Wnt receptor antibody
      • Xfrizzled-1 antibody
      see all

    Images

    • Western blot - Anti-Frizzled homolog 1 antibody (ab117305)
      Western blot - Anti-Frizzled homolog 1 antibody (ab117305)
      All lanes : Anti-Frizzled homolog 1 antibody (ab117305) at 1 µg/ml

      Lane 1 : Human heart tissue lysate - total protein (ab29431)
      Lane 2 : Human placenta tissue lysate - total protein (ab29745)
      Lane 3 : Lung (Human) Tissue Lysate
      Lane 4 : Human kidney tissue lysate - total protein (ab30203)
      Lane 5 : Human pancreas tissue lysate - total protein (ab29816)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 71 kDa
      Observed band size: 71 kDa
      Additional bands at: 40 kDa, 47 kDa, 88 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 6 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117305 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab117305? Please let us know so that we can cite the reference in this datasheet.

    ab117305 has not yet been referenced specifically in any publications.

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