Anti-Frizzled homolog 1 antibody (ab117305)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Frizzled homolog 1 antibody
See all Frizzled homolog 1 primary antibodies -
Description
Rabbit polyclonal to Frizzled homolog 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
-
Immunogen
Synthetic peptide corresponding to Human Frizzled homolog 1 aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab150442) -
Positive control
- This antibody gave a positive signal in the following Human tissue lysates: Heart; Placenta; Lung; Kidney; Pancreas.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab117305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
Target
-
Function
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B. -
Tissue specificity
Expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney. -
Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
Domain
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
Cellular localization
Membrane. Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 445417 Cow
- Entrez Gene: 482294 Dog
- Entrez Gene: 101126536 Gorilla
- Entrez Gene: 100060569 Horse
- Entrez Gene: 8321 Human
- Entrez Gene: 14362 Mouse
- Entrez Gene: 58868 Rat
- Omim: 603408 Human
see all -
Alternative names
- Chfz-1 antibody
- Dfrizzled-1 antibody
- DKFZp564G072 antibody
see all
Images
-
Western blot - Anti-Frizzled homolog 1 antibody (ab117305)All lanes : Anti-Frizzled homolog 1 antibody (ab117305) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lane 3 : Lung (Human) Tissue Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203)
Lane 5 : Human pancreas tissue lysate - total protein (ab29816)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Additional bands at: 40 kDa, 47 kDa, 88 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 6 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117305 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References
ab117305 has not yet been referenced specifically in any publications.
