Overview

  • Product name

  • Description

    Rabbit polyclonal to FRK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of Human FRK

  • Positive control

    • Human breast carcinoma tissue, HUVEC cells and extracts from Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab64914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function

    Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
  • Tissue specificity

    Restricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta cell Src homology tyrosine kinase antibody
    • BSK antibody
    • BSK/IYK antibody
    • C85044 antibody
    • Frk antibody
    • Frk fyn related kinase antibody
    • FRK_HUMAN antibody
    • FYN related kinase antibody
    • FYN-related kinase antibody
    • GASK antibody
    • Gastrointestinal associated kinase antibody
    • Gastrointestinal associated tyrosine kinase antibody
    • GTK antibody
    • GTK, mouse, homolog of antibody
    • Intestine tyrosine kinase antibody
    • Nuclear tyrosine protein kinase RAK antibody
    • Protein-tyrosine kinase 5 antibody
    • PTK5 antibody
    • PTK5 protein tyrosine kinase 5 antibody
    • RAK antibody
    • Tyrosine protein kinase FRK antibody
    • Tyrosine-protein kinase FRK antibody
    see all

Images

  • All lanes : Anti-FRK antibody (ab64914) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 56 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?

  • ab64914, at a 1/50 dilution, staining Human FRK in paraffin embedded breast carcinoma tissue, by Immunohistochemistry, with (+) or without (-) immunising peptide.
  • ab64914, at a 1/500 dilution, staining Human FRK in HUVEC cells, by Immunofluorescence, with (+) or without (-) immunising peptide.

References

This product has been referenced in:

  • Yumnam S  et al. Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array. Mol Med Rep 16:5386-5392 (2017). Read more (PubMed: 28849065) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Stomach)
Specification
Stomach
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 01 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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