Key features and details
- Rabbit polyclonal to FRK
- Suitable for: ELISA, WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FRK antibody
See all FRK primary antibodies
DescriptionRabbit polyclonal to FRK
Tested applicationsSuitable for: ELISA, WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from an internal sequence of Human FRK
- Human breast carcinoma tissue, HUVEC cells and extracts from Jurkat cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).|
|ICC/IF||1/500 - 1/1000.|
|IHC-P||1/50 - 1/100.|
FunctionPositively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
Tissue specificityRestricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
- Information by UniProt
- Beta cell Src homology tyrosine kinase antibody
- BSK antibody
- BSK/IYK antibody
All lanes : Anti-FRK antibody (ab64914) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 56 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
ab64914, at a 1/50 dilution, staining Human FRK in paraffin embedded breast carcinoma tissue, by Immunohistochemistry, with (+) or without (-) immunising peptide.
ab64914, at a 1/500 dilution, staining Human FRK in HUVEC cells, by Immunofluorescence, with (+) or without (-) immunising peptide.
ab64914 has been referenced in 1 publication.
- Yumnam S et al. Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array. Mol Med Rep 16:5386-5392 (2017). PubMed: 28849065