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Rabbit polyclonal to FRMD4A
antigen sequence: EGAHDKGAGR AAVSDELRQW YQRSTASHKE HSRLSHTSST SSDSGSQYST SSQSTFVAHS RVTRMPQMCK ATSA, corresponding to amino acids 912-985 of Human FRMD4A.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 1 FERM domain.
Cytoplasm > cytoskeleton.
Information by UniProt
- bA295P9.4 antibody
- FERM domain containing 4A antibody
- FERM domain-containing protein 4A antibody
Immunocytochemistry/ Immunofluorescence - Anti-FRMD4A antibody (ab122475)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and golgi apparatus. Recommended concentration of ab122475 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (ab122475)
ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human testis tissue by Immunohistochemistry.
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