Key features and details
- Rabbit polyclonal to FRMD5
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FRMD5 antibody
DescriptionRabbit polyclonal to FRMD5
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human FRMD5 aa 295-504.
Database link: Q7Z6J6
- WB: Mouse kidney, heart, skeletal muscle and lung tissue lysates. IHC-P: Human glioma and kidney tissues. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 66, 64 kDa.|
Sequence similaritiesContains 1 FERM domain.
- Information by UniProt
- FERM domain containing 5 antibody
- FERM domain-containing protein 5 antibody
- FLJ41022 antibody
All lanes : Anti-FRMD5 antibody (ab229932) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lane 4 : Mouse lung tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 66, 64 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Paraffin-embedded human glioma tissue stained for FRMD5 using ab229932 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for FRMD5 using ab229932 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FRMD5 (green) using ab229932 at 1/100 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat anti-Rabbit IgG (H+L) as secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229932 has not yet been referenced specifically in any publications.