Overview

  • Product name

    Anti-FRMD5 antibody
  • Description

    Rabbit polyclonal to FRMD5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human FRMD5 aa 295-504.
    Database link: Q7Z6J6

  • Positive control

    • WB: Mouse kidney, heart, skeletal muscle and lung tissue lysates. IHC-P: Human glioma and kidney tissues. ICC/IF: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Predicted molecular weight: 66, 64 kDa.

Target

Images

  • All lanes : Anti-FRMD5 antibody (ab229932) at 1/1000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse skeletal muscle tissue lysate
    Lane 4 : Mouse lung tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 66, 64 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded human glioma tissue stained for FRMD5 using ab229932 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human kidney tissue stained for FRMD5 using ab229932 at 1/100 dilution in immunohistochemical analysis.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FRMD5 (green) using ab229932 at 1/100 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat anti-Rabbit IgG (H+L) as secondary antibody.

References

ab229932 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229932.
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