Anti-FRMD6/Willin antibody (ab110675)
Key features and details
- Rabbit polyclonal to FRMD6/Willin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-FRMD6/Willin antibody
See all FRMD6/Willin primary antibodies -
Description
Rabbit polyclonal to FRMD6/Willin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in U20S whole cell lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: A431.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Sequence similarities
Contains 1 FERM domain. -
Cellular localization
Cytoplasm. Cell membrane. Can colocalize with actin. - Information by UniProt
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Database links
- Entrez Gene: 122786 Human
- Entrez Gene: 319710 Mouse
- Entrez Gene: 257646 Rat
- Omim: 614555 Human
- SwissProt: Q96NE9 Human
- SwissProt: Q8C0V9 Mouse
- SwissProt: Q8VII0 Rat
- Unigene: 434914 Human
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Alternative names
- C14orf31 antibody
- EX1 antibody
- FERM domain-containing protein 6 antibody
see all
Images
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ICC/IF image of ab110675 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110675 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Anti-FRMD6/Willin antibody (ab110675) at 1 µg/ml (Milk blocking - 3%) + U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab110675 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab110675 has been referenced in 1 publication.
- Zhang Y et al. Inhibin βA is an independent prognostic factor that promotes invasion via Hippo signaling in non‑small cell lung cancer. Mol Med Rep 24:N/A (2021). PubMed: 34505633