Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12261] to FRMD6/Willin
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-FRMD6/Willin antibody [EPR12261]
See all FRMD6/Willin primary antibodies
DescriptionRabbit monoclonal [EPR12261] to FRMD6/Willin
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
- Human fetal liver and A431 lysates; A431 cells and permeabilized A431 cells.
This product was previously labelled as FRMD6
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 72 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Sequence similaritiesContains 1 FERM domain.
Cellular localizationCytoplasm. Cell membrane. Can colocalize with actin.
- Information by UniProt
- C14orf31 antibody
- EX1 antibody
- FERM domain-containing protein 6 antibody
Immunofluorescence analysis of A431 cells labeling FRMD6/Willin using ab171745 at 1/100 dilution.
Flow cytometric analysis of permeabilized A431 cells labeling FRMD6/Willin using ab171745 (red) at 1/100 dilution or a rabbit IgG (negative) (green).
All lanes : Anti-FRMD6/Willin antibody [EPR12261] (ab171745) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa
ab171745 has been referenced in 2 publications.
- Zhang X et al. Notch3 inhibits epithelial-mesenchymal transition by activating Kibra-mediated Hippo/YAP signaling in breast cancer epithelial cells. Oncogenesis 5:e269 (2016). PubMed: 27841855
- Jeibmann A et al. Identification of genes involved in the biology of atypical teratoid/rhabdoid tumours using Drosophila melanogaster. Nat Commun 5:4005 (2014). PubMed: 24892285