Anti-FRMD8 antibody (ab219857)
Key features and details
- Rabbit polyclonal to FRMD8
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FRMD8 antibody
See all FRMD8 primary antibodies -
Description
Rabbit polyclonal to FRMD8 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human FRMD8 aa 300-450.
Database link: Q9BZ67 -
Positive control
- RT-4 and U-251 MG cell lysates; Human tonsil tissue lysate; U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 51 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 51 kDa. |
Target
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Sequence similarities
Contains 1 FERM domain. - Information by UniProt
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Database links
- Entrez Gene: 83786 Human
- Entrez Gene: 67457 Mouse
- Entrez Gene: 309172 Rat
- SwissProt: Q9BZ67 Human
- SwissProt: Q3UFK8 Mouse
- SwissProt: Q5U2R3 Rat
- Unigene: 578433 Human
- Unigene: 202092 Mouse
see all -
Alternative names
- FERM domain containing 8 antibody
- FERM domain containing protein 8 antibody
- FERM domain-containing protein 8 antibody
see all
Images
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Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-251 MG cells labeling FRMD8 cells with ab219857 at 4 µg/ml (green).
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All lanes : Anti-FRMD8 antibody (ab219857) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 51 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab219857 has not yet been referenced specifically in any publications.