Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14724] to FRS2
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
Product nameAnti-FRS2 antibody [EPR14724]
See all FRS2 primary antibodies
DescriptionRabbit monoclonal [EPR14724] to FRS2
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment within Human FRS2 aa 100-250. The exact sequence is proprietary.
Database link: Q8WU20
- Human fetal brain, HeLa and SH-SY5Y lysates; SH-SY5Y cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab183492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 60-90 kDa (predicted molecular weight: 57 kDa).|
|IP||1/50 - 1/70.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAdapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
Tissue specificityHighly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.
Sequence similaritiesContains 1 IRS-type PTB domain.
modificationsPhosphorylated on tyrosine residues upon stimulation by NGF.
Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination.
Cellular localizationEndomembrane system. Cytoplasmic, membrane-bound.
- Information by UniProt
- FGFR signaling adaptor SNT antibody
- FGFR signalling adaptor antibody
- FGFR substrate 2 antibody
All lanes : Anti-FRS2 antibody [EPR14724] (ab183492) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa
Anti-FRS2 antibody [EPR14724] (ab183492) at 1/2000 dilution + SH-SY5Y cell lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa
Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling FRS2 with ab183492 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of FRS2 in Human fetal brain lysate immunoprecipitated using ab183492 at 1/50 dilution (Lane 1). Lane 2: TBS instead of Human fetal brain lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
ab183492 has been referenced in 1 publication.
- Goyal L et al. TAS-120 Overcomes Resistance to ATP-Competitive FGFR Inhibitors in Patients with FGFR2 Fusion-Positive Intrahepatic Cholangiocarcinoma. Cancer Discov 9:1064-1079 (2019). PubMed: 31109923