Overview

  • Product name

    Anti-FRS2 antibody [EPR14724]
    See all FRS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14724] to FRS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human FRS2 aa 100-250. The exact sequence is proprietary.
    Database link: Q8WU20

  • Positive control

    • Human fetal brain, HeLa and SH-SY5Y lysates; SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14724
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60-90 kDa (predicted molecular weight: 57 kDa).
IP 1/50 - 1/70.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Adapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
  • Tissue specificity

    Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.
  • Sequence similarities

    Contains 1 IRS-type PTB domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues upon stimulation by NGF.
    Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination.
  • Cellular localization

    Endomembrane system. Cytoplasmic, membrane-bound.
  • Information by UniProt
  • Database links

  • Alternative names

    • FGFR signaling adaptor SNT antibody
    • FGFR signalling adaptor antibody
    • FGFR substrate 2 antibody
    • FGFR-signaling adaptor SNT antibody
    • Fibroblast growth factor receptor substrate 2 antibody
    • FRS 2 antibody
    • FRS2 antibody
    • FRS2_HUMAN antibody
    • FRS2A antibody
    • FRS2alpha antibody
    • SNT 1 antibody
    • SNT antibody
    • SNT-1 antibody
    • SNT1 antibody
    • Suc 1 Associated Neurotrophic Factor Target antibody
    • Suc1 associated neurotrophic factor target 1 antibody
    • Suc1-associated neurotrophic factor target 1 antibody
    see all

Images

  • All lanes : Anti-FRS2 antibody [EPR14724] (ab183492) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 57 kDa

  • Anti-FRS2 antibody [EPR14724] (ab183492) at 1/2000 dilution + SH-SY5Y cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 57 kDa

  • Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling FRS2 with ab183492 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Western blot analysis of FRS2 in Human fetal brain lysate immunoprecipitated using ab183492 at 1/50 dilution (Lane 1). Lane 2: TBS instead of Human fetal brain lysate.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab183492 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183492.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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