Fructose-6-Phosphate Assay Kit (Fluorometric) (ab204720)

Overview

  • Product name

    Fructose-6-Phosphate Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Cell Lysate, Tissue Lysate
  • Sensitivity

    1 µM
  • Product overview

    Fructose-6-Phosphate Assay Kit (ab204720) is a fluorescence based simple, highly sensitive and rapid means of quantifying fructose-6-phosphate (F6P) in a variety of samples. In the assay, F6P is converted to glucose-6-phosphate (G6P) which is subsequently oxidized with the generation of a fluorescent product. This assay kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity ∼ 1 µM of F6P.

  • Notes

    Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    F6P Assay Buffer 1 x 25ml
    F6P Converter (lyophilized) 1 vial
    F6P Enzyme Mix (lyophilized) 1 vial
    F6P Probe 1 x 400µl
    F6P Standard (10 umol, lyophilized) 1 vial
    F6P Substrate Mix (lyophilized) 1 vial

Images

  • Typical F6P Standard calibration curve

  • Typical F6P Standard calibration curve

Protocols

References

ab204720 has not yet been referenced specifically in any publications.

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