Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 1 µM
Product nameFructose-6-Phosphate Assay Kit (Fluorometric)
Sample typeCell Lysate, Tissue Lysate
Fructose-6-Phosphate Assay Kit (ab204720) is a fluorescence based simple, highly sensitive and rapid means of quantifying fructose-6-phosphate (F6P) in a variety of samples. In the assay, F6P is converted to glucose-6-phosphate (G6P) which is subsequently oxidized with the generation of a fluorescent product. This assay kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity ∼ 1 µM of F6P.
Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests F6P Assay Buffer 1 x 25ml F6P Converter (lyophilized) 1 vial F6P Enzyme Mix (lyophilized) 1 vial F6P Probe 1 x 400µl F6P Standard (10 umol, lyophilized) 1 vial F6P Substrate Mix (lyophilized) 1 vial
ab204720 has not yet been referenced specifically in any publications.