Overview

  • Product name

    Fructose Assay Kit
    See all Fructose kits
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Quantitative
  • Assay time

    1h 00m
  • Product overview

    In Abcam's Fructose Assay Kit, free fructose is enzymatically converted to ß-glucose, which is then specifically converted to a product that reacts with OxiRed Probe to generate color (λ=570nm) and fluorescence (Ex/Em=535/587nm). The kit provides a rapid, simple, sensitive, and reliable method suitable for high throughput assayof D-fructose. Do not use this kit with urine, serum, or plasma samples. 
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Enzyme Mix (lyophilized) Green 1 vial
    Fructose Assay Buffer WM 1 x 25ml
    Fructose Converting Enzyme (Lyophilised) Purple 1 vial
    Fructose Standard (100mM) Yellow 1 x 100µl
    OxiRed Probe in DMSO Red 1 x 200µl
  • Research areas

  • Relevance

    Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose.
  • Alternative names

    • Beta Levulose
    • D arabino Hexulose
    • Fruit sugar
    • Laevulose
    • Levulose
    see all

Images

  • Fructose levels banana, determined using the colorimetric (C) and the fluorometric (F) method (presented per mg of protein) (duplicates; +/- SD).

  • Fructose levels in mouse liver and banana, determined using the colorimetric method and presented per mg of protein (duplicates; +/- SD).

  • Titration of the fructose (background subtracted, duplicates; +/- SD).

  • Fluorometric Fructose Standard Curve performed following the ab83380 kit protocol.

Protocols

References

This product has been referenced in:

  • Maslova O  et al. Production of various organic acids from different renewable sources by immobilized cells in the regimes of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SFF). Bioresour Technol 272:1-9 (2019). Read more (PubMed: 30292911) »
  • Aw WC  et al. Genotype to phenotype: Diet-by-mitochondrial DNA haplotype interactions drive metabolic flexibility and organismal fitness. PLoS Genet 14:e1007735 (2018). Read more (PubMed: 30399141) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Yes, samples made in RIPA buffer can be used, albeit, they need to be diluted in the respective assays buffers before use with the particular assays, so that the samples have all the components required for each reaction to occur.

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Answer

Thank you for contacting us. The kit is currently priced at $435 and is for 100 tests. The protocol calls for 6 readings of standards, so if you take into account doing everything in duplicate (standards and samples) that would be 44 different samples. Please let me know if you have any further questions.

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Answer

Thank you for your inquiry and for notifying us of the missing protocol.

The protocol has now been added to the datasheet and I also attached a copy for your customer to this email.

Please do not hesitate to contact us again with any further questions.

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Answer

Thank you for your reply. We only have the full size kit available for purchase. However we guarantee all our products to work as stated on the datasheets, so we will be happy to help if the kit does not work as expected. Please let me know if you have any questions, or if there is anything else we can help with.

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Question
Answer

Thanks for your call yesterday and for your question about ab83380. I have been in contact with the lab, and I was told that the detection limit when the kit is used colorimetrically (570nm) is 1-10 nmols/well. If fluorometric methods (Ex/Em = 535/587nm) are chosen, the sensitivity increases by 10-100 fold (0.1- 1.0 nmols/well). I hope this information is useful, but please let me know if you have any further questions and I'll be happy to help.

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