Overview

  • Product name

    Fructose Assay Kit (Fluorometric)
    See all Fructose kits
  • Detection method

    Fluorescent
  • Product overview

    Fructose Assay Kit (Fluorometric) (ab241022) measures free fructose that is enzymatically processed with the formation of a metabolite which reacts with the Probe to generate fluorescence (Ex/Em = 535/587 nm). The kit provides a simple, highly sensitive, reliable method suitable for high throughput assay of D-fructose. Glucose interference can be removed by using the Sample Cleanup Mix. The kit can detect fructose in the range of 5 to 500 picomoles/well.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Conversion Enzyme 1 vial
    Fructose Assay Buffer 1 x 25ml
    Fructose Enzyme Mix 1 vial
    Fructose Standard (100 mM) 1 x 100µl
    Fructose Substrate Mix 1 vial
    Probe 1 x 200µl
    Sample Cleanup Mix 1 vial
  • Research areas

  • Relevance

    Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose.
  • Alternative names

    • Beta Levulose
    • D arabino Hexulose
    • Fruit sugar
    • Laevulose
    • Levulose
    see all

Images

  • Fructose standard curve generated using this kit protocol.
  • Fructose standard curve generated using this kit protocol.

Protocols

References

ab241022 has not yet been referenced specifically in any publications.

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