Product nameAnti-FRYL antibody
See all FRYL primary antibodies
DescriptionRabbit polyclonal to FRYL
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
- HeLa and 293T Whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab95011 was affinity purified using an epitope specific to FRYL immobilized on solid support.
Our Abpromise guarantee covers the use of ab95011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 340 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPlays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.
Tissue specificityWidely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.
Involvement in diseaseA chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.
Sequence similaritiesBelongs to the furry protein family.
- Information by UniProt
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All lanes : Anti-FRYL antibody (ab95011) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 340 kDa
Exposure time: 3 minutes
ab95011, at 1 µg/ml, detecting FRYL in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of FRYL
Lane 2: ab95011 at 3 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds
ab95011 has not yet been referenced specifically in any publications.