• Product name
  • Description
    Rabbit polyclonal to FRYL
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 2600-2650 of Human FRYL (NP_055845.1).

  • Positive control
    • HeLa whole cell lysate (ab150035), 293T cell lysate, Mouse NIH3T3 cell lysate,


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype


Our Abpromise guarantee covers the use of ab95065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 340 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function
    Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.
  • Tissue specificity
    Widely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.
  • Involvement in disease
    A chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.
  • Sequence similarities
    Belongs to the furry protein family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010313D22Rik antibody
    • 2310004H21Rik antibody
    • 2510002A14Rik antibody
    • 9030227G01Rik antibody
    • AF4P12 antibody
    • AI930088 antibody
    • ALL fused gene from chromosome 4p12 antibody
    • ALL1-fused gene from chromosome 4p12 protein antibody
    • DKFZp686E205 antibody
    • FLJ161772 antibody
    • FRY like antibody
    • FRYL antibody
    • FRYL_HUMAN antibody
    • Furry homolog like (Drosophila) antibody
    • Furry homolog like antibody
    • Furry like antibody
    • KIAA0826 antibody
    • mKIAA0826 antibody
    • Protein furry homolog-like antibody
    see all


  • All lanes : Anti-FRYL antibody (ab95065) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 340 kDa

    Exposure time: 10 seconds
  • Lane 1: Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
    Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
    Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.

    Subsequent Western blot detection used ab95065 at 1 µg/ml.

    Developed using the ECL technique with an exposure time of 1 seconds.


This product has been referenced in:
  • Rippe C  et al. Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway. Sci Rep 7:1334 (2017). WB ; Mouse . Read more (PubMed: 28465505) »
  • Peterson SM  et al. Global gene expression analysis reveals dynamic and developmental stage-dependent enrichment of lead-induced neurological gene alterations. Environ Health Perspect 119:615-21 (2011). Read more (PubMed: 21147602) »
See all 2 Publications for this product

Customer reviews and Q&As


I will be happy to help you with this.

Our datasheet (https://www.abcam.com/fryl-antibody-ab95065.html) shows data similar to what your are seeing: many bands at lower molecular weights but also a single band at the expected size. These samples are cell lysates, and I think your samples, from cortex, are going to be more problematic for this antibody, which cross-reacts with other proteins. My guess is that the amount of Fryl in the cell line lysates as a proportion of total protein may make it easier to isolate a Fryl signal in blots of these lysates than in blots of your samples, if Fryl is present in the synaptosomes as a smaller proportion of total protein than in these cell lines.

Farther down the datasheet , another blot of HeLa lysates suggests that it is necessary to IP the protein in order to see a clean Fryl signal. But it is not clear to me that your blots show any signal for Fryl, so I cannot say IP will help you.

The laboratory that validated the antibody for western blotting used HiMark molecular weight markers and NuPage 3-8% Tris-acetate gels (cat# EA03752BOX) with the recommended buffers (all from Invitrogen).

My advice is to optimize the transfer for large proteins. I recommend removing methanol from the transfer buffer and including SDS at a concentration of 0.1%, to encourage movement of Fryl out of the gel, onto PVDF.

We have one other Fryl antibody, ab95011, raised against a different region, but the WB data looks very similar, with some cross-reactivity at lower molecular weights. I will be happy to send this a free replacement if you want to try it, or a credit or refund.

I look forward to your reply.

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