Anti-FRYL antibody (ab95065)

Reviews (3)Q&A (1)References (3)
Western blot - Anti-FRYL antibody (ab95065)
  • Immunoprecipitation - Anti-FRYL antibody (ab95065)

Key features and details

  • Rabbit polyclonal to FRYL
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-FRYL antibody
    See all FRYL primary antibodies

  • Description

    Rabbit polyclonal to FRYL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 2600-2650 of Human FRYL (NP_055845.1).

  • Positive control

    • HeLa whole cell lysate (ab150035), 293T cell lysate, Mouse NIH3T3 cell lysate,

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab95065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 340 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.

  • Tissue specificity

    Widely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.

  • Involvement in disease

    A chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.

  • Sequence similarities

    Belongs to the furry protein family.
  • Information by UniProt

  • Database links

    • Alternative names

      • 2010313D22Rik antibody
      • 2310004H21Rik antibody
      • 2510002A14Rik antibody
      • 9030227G01Rik antibody
      • AF4P12 antibody
      • AI930088 antibody
      • ALL fused gene from chromosome 4p12 antibody
      • ALL1-fused gene from chromosome 4p12 protein antibody
      • DKFZp686E205 antibody
      • FLJ161772 antibody
      • FRY like antibody
      • FRYL antibody
      • FRYL_HUMAN antibody
      • Furry homolog like (Drosophila) antibody
      • Furry homolog like antibody
      • Furry like antibody
      • KIAA0826 antibody
      • mKIAA0826 antibody
      • Protein furry homolog-like antibody
      see all

    Images

    • Western blot - Anti-FRYL antibody (ab95065)
      Western blot - Anti-FRYL antibody (ab95065)
      All lanes : Anti-FRYL antibody (ab95065) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg
      Lane 5 : Mouse NIH3T3 cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 340 kDa


      Exposure time: 10 seconds
    • Immunoprecipitation - Anti-FRYL antibody (ab95065)
      Immunoprecipitation - Anti-FRYL antibody (ab95065)
      Lane 1: Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
      Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
      Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.

      Subsequent Western blot detection used ab95065 at 1 µg/ml.

      Developed using the ECL technique with an exposure time of 1 seconds.

    References (3)

    Publishing research using ab95065? Please let us know so that we can cite the reference in this datasheet.

    ab95065 has been referenced in 3 publications.

    • Yeung ATY  et al. A Genome-Wide Knockout Screen in Human Macrophages Identified Host Factors Modulating Salmonella Infection. mBio 10:N/A (2019). PubMed: 31594818
    • Rippe C  et al. Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway. Sci Rep 7:1334 (2017). WB ; Mouse . PubMed: 28465505
    • Peterson SM  et al. Global gene expression analysis reveals dynamic and developmental stage-dependent enrichment of lead-induced neurological gene alterations. Environ Health Perspect 119:615-21 (2011). PubMed: 21147602

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-FRYL antibody

     Excellent
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Non-tumorigenic and tumorigenic prostate cancer ce)
    Total protein in input
    500 µg
    Treatment
    Transfection with inhibitor
    Immuno-precipitation step
    Other - Protein A Dynabeads
    Specification
    Non-tumorigenic and tumorigenic prostate cancer ce
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 30 2020

    Western blot abreview for Anti-FRYL antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (lung cancer, NSCLC (A549))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    lung cancer, NSCLC (A549)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 26 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FRYL antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (lung cancer, NSCLC (A549))
    Permeabilization
    Yes - triton-x
    Specification
    lung cancer, NSCLC (A549)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 26 2020

    Question

    I ordered 3 antibodies from Abcam on 1/3/12, and 2 of them worked quite well for western blot. However, Rb-anti-Fryl (ab95065) did not work in my western blots and I am wondering if you can provide troubleshooting suggestions or a refund.


    This antibody is supposed to recognize a band at 340 kDa and has been shown to detect mouse Fryl. My samples are synaptosomes prepared from mouse cortex and lysed for western blot. I used this antibody at 1:2,000 in either 3% BSA + azide or 1% milk. I also tried boiling the lysate or only heating it to 37C. I am attaching the results I obtained from these western blots (I tried 0.2 ug, 1 ug, and 5 ug per lane).

    Read More
    Answer

    I will be happy to help you with this.

    Our datasheet (https://www.abcam.com/fryl-antibody-ab95065.html) shows data similar to what your are seeing: many bands at lower molecular weights but also a single band at the expected size. These samples are cell lysates, and I think your samples, from cortex, are going to be more problematic for this antibody, which cross-reacts with other proteins. My guess is that the amount of Fryl in the cell line lysates as a proportion of total protein may make it easier to isolate a Fryl signal in blots of these lysates than in blots of your samples, if Fryl is present in the synaptosomes as a smaller proportion of total protein than in these cell lines.

    Farther down the datasheet , another blot of HeLa lysates suggests that it is necessary to IP the protein in order to see a clean Fryl signal. But it is not clear to me that your blots show any signal for Fryl, so I cannot say IP will help you.

    The laboratory that validated the antibody for western blotting used HiMark molecular weight markers and NuPage 3-8% Tris-acetate gels (cat# EA03752BOX) with the recommended buffers (all from Invitrogen).

    My advice is to optimize the transfer for large proteins. I recommend removing methanol from the transfer buffer and including SDS at a concentration of 0.1%, to encourage movement of Fryl out of the gel, onto PVDF.

    We have one other Fryl antibody, ab95011, raised against a different region, but the WB data looks very similar, with some cross-reactivity at lower molecular weights. I will be happy to send this a free replacement if you want to try it, or a credit or refund.

    I look forward to your reply.

    Read More

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