Anti-FRYL antibody (ab95065)
Key features and details
- Rabbit polyclonal to FRYL
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FRYL antibody -
Description
Rabbit polyclonal to FRYL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 2600-2650 of Human FRYL (NP_055845.1).
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Positive control
- HeLa whole cell lysate (ab150035), 293T cell lysate, Mouse NIH3T3 cell lysate,
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 340 kDa.
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IP | (1) |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 340 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator. -
Tissue specificity
Widely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin. -
Involvement in disease
A chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein. -
Sequence similarities
Belongs to the furry protein family. - Information by UniProt
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Database links
- Entrez Gene: 285527 Human
- Entrez Gene: 72313 Mouse
- Entrez Gene: 364144 Rat
- SwissProt: O94915 Human
- SwissProt: E9Q8I9 Mouse
- Unigene: 595553 Human
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Alternative names
- 2010313D22Rik antibody
- 2310004H21Rik antibody
- 2510002A14Rik antibody
see all
Images
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All lanes : Anti-FRYL antibody (ab95065) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 340 kDa
Exposure time: 10 seconds -
Lane 1: Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab95065 at 1 µg/ml.
Developed using the ECL technique with an exposure time of 1 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab95065 has been referenced in 3 publications.
- Yeung ATY et al. A Genome-Wide Knockout Screen in Human Macrophages Identified Host Factors Modulating Salmonella Infection. mBio 10:N/A (2019). PubMed: 31594818
- Rippe C et al. Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway. Sci Rep 7:1334 (2017). WB ; Mouse . PubMed: 28465505
- Peterson SM et al. Global gene expression analysis reveals dynamic and developmental stage-dependent enrichment of lead-induced neurological gene alterations. Environ Health Perspect 119:615-21 (2011). PubMed: 21147602