Recombinant Anti-FRZB antibody [EPR23904-20] (ab273582)
![Western blot - Anti-FRZB antibody [EPR23904-20] (ab273582)](https://www.abcam.com/ps/products/273/ab273582/Images/ab273582-394328-anti-frzb-antibody-epr2390420-western-blot-mouse-brain-tissue-lysate-20-ug.jpg "Western blot - Anti-FRZB antibody [EPR23904-20] (ab273582)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23904-20] to FRZB
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-FRZB antibody [EPR23904-20]
See all FRZB primary antibodies -
Description
Rabbit monoclonal [EPR23904-20] to FRZB -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, ICC/IF, WBmore details
Unsuitable for: IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and testis tissue lysates. ICC/IF: Neuro-2a cells. Flow Cyt (intra): Neuro-2a cells. IP: Mouse testis tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23904-20 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
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| IP |
1/30.
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| ICC/IF |
1/50.
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| WB |
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. |
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IP
1/30. |
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ICC/IF
1/50. |
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WB
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Target
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Relevance
SFRP3/FRZB appears to be involved in limb skeletogenesis by regulating chondrocyte maturation and long bone development. Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. Antagonist of Wnt8 signaling. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 20378 Mouse
- SwissProt: P97401 Mouse
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Alternative names
- FIZ antibody
- FRE antibody
- Frezzled antibody
see all
Images
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Western blot - Anti-FRZB antibody [EPR23904-20] (ab273582)All lanes : Anti-FRZB antibody [EPR23904-20] (ab273582) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28300015).
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-FRZB antibody [EPR23904-20] (ab273582)](https://www.abcam.com/ps/products/273/ab273582/Images/ab273582-3-anti-frzb-antibody-epr2390420-immunoprecipitation-mouse-testis-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-FRZB antibody [EPR23904-20] (ab273582)FRZB was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab273582 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273582 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse testis tissue lysate 10 ug
Lane 2: ab273582 IP in Mouse testis tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273582 in mouse testis tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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![Flow Cytometry (Intracellular) - Anti-FRZB antibody [EPR23904-20] (ab273582)](https://www.abcam.com/ps/products/273/ab273582/Images/ab273582-2-anti-frzb-antibody-epr2390420-flow-cytometry-neuro2a.jpg)
Flow Cytometry (Intracellular) - Anti-FRZB antibody [EPR23904-20] (ab273582)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling FRZB with ab273582 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-FRZB antibody [EPR23904-20] (ab273582)](https://www.abcam.com/ps/products/273/ab273582/Images/ab273582-1-anti-frzb-antibody-epr2390420-immunocytochemistry-neuro2a.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FRZB antibody [EPR23904-20] (ab273582)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling FRZB with ab273582 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Anti-FRZB antibody [EPR23904-20] (ab273582)](https://www.abcam.com/ps/products/273/ab273582/Images/ab273582-5-benefits-of-recombinant-antibodies.png)
Anti-FRZB antibody [EPR23904-20] (ab273582)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab273582 has been referenced in 1 publication.
- Xu R et al. Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip. Aging (Albany NY) 13:11281-11295 (2021). PubMed: 33820867