Product nameAnti-FSCB antibody
See all FSCB primary antibodies
DescriptionRabbit polyclonal to FSCB
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human Liver and Tonsil lysates; Human testis tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
FunctionMay be involved in the later stages of fibrous sheath biogenesis. Binds calcium.
Cellular localizationCell projection > cilium > flagellum. Localizes to cortex of the fibrous sheath including the surface of the longitudinal columns and ribs of the principal piece of sperm flagella.
- Information by UniProt
- C14orf155 antibody
- Fibrous sheath CABYR-binding protein antibody
- Fscb antibody
- FSCB_HUMAN antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab121201 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-FSCB antibody (ab121201) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
ab121201 at 1/300 dilution staining FSCB in Paraffin-emebedded Human testis tissue by Immunohistochemistry.
ab121201 has not yet been referenced specifically in any publications.