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Rabbit polyclonal to FSCB
Recombinant fragment, corresponding to amino acids 721-807 (ADLLLTEEFP IGEASAEVSP PPSEQTPEDE ALVENVSTEF QSPQVAGIPA VKLGSVVLEG EAKFEEVSKI NSVLKDLSNT NDGQAPT) of Human FSCB (Q5H9T9).
- Human Liver and Tonsil lysates; Human testis tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
May be involved in the later stages of fibrous sheath biogenesis. Binds calcium.
Cell projection > cilium > flagellum. Localizes to cortex of the fibrous sheath including the surface of the longitudinal columns and ribs of the principal piece of sperm flagella.
Information by UniProt
- C14orf155 antibody
- Fibrous sheath CABYR-binding protein antibody
- Fscb antibody
- FSCB_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-FSCB antibody (ab121201)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab121201 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-FSCB antibody (ab121201)
All lanes : Anti-FSCB antibody (ab121201) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSCB antibody (ab121201)
ab121201 at 1/300 dilution staining FSCB in Paraffin-emebedded Human testis tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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