Overview

  • Product name

    Anti-FSD1 antibody
  • Description

    Rabbit polyclonal to FSD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human FSD1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9BTV5

  • Positive control

    • WB: U87-MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IHC-P: Mouse brain. ICC/IF: SK-N-SH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1200.
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.

Target

  • Function

    May be involved in microtubule organization and stabilization.
  • Tissue specificity

    Highly expressed in brain tissues, including cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Lower expression in spinal chord.
  • Sequence similarities

    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
  • Domain

    B30.2 box contains a microtubule-binding site.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Nucleus. Cytoplasm. Cleavage furrow. Cell-cycle-dependent association with the centrosome. Colocalizes with a subpopulation of microtubules. Does not associates with microtubules during mitosis but reassociates with microtubules during cytokinesis. Localizes to the central portions of a small subset of microtubules in interphase cells and a subpopulation of microtubules in the cleavage furrow, not present in the mitotic spindle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fibronectin type 3 and SPRY (spla, ryanodine) domain containing (with coiled-coil motif) 1 antibody
    • Fibronectin type 3 and SPRY domain containing 1 antibody
    • Fibronectin type III and SPRY domain containing 1 antibody
    • Fibronectin type III and SPRY domain-containing protein 1 antibody
    • Fibronectin type III and SPRY domains containing protein 1 antibody
    • FSD1 antibody
    • FSD1_HUMAN antibody
    • GLFND antibody
    • MGC3213 antibody
    • MGC58341 antibody
    • Microtubule-associated protein GLFND antibody
    • MID1-related protein 1 antibody
    • midline 1-related protein 1 antibody
    • MIR1 antibody
    • VLP27 antibody
    see all

Images

  • 4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for FSD1 (green) using ab229666 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded mouse brain tissue stained for FSD1 using ab229666 at 1/1200 dilution in immunohistochemical analysis.

  • All lanes : Anti-FSD1 antibody (ab229666) at 1/1000 dilution

    Lane 1 : U87-MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 (human neuroblast cell line) whole cell extract
    Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 56 kDa



    10% SDS-PAGE gel.

References

ab229666 has not yet been referenced specifically in any publications.

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