Key features and details
- Rabbit polyclonal to FSD1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FSD1 antibody
DescriptionRabbit polyclonal to FSD1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant fragment within Human FSD1 (internal sequence). The exact sequence is proprietary.
Database link: Q9BTV5
- WB: U87-MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IHC-P: Mouse brain. ICC/IF: SK-N-SH cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1200.|
|WB||1/500 - 1/3000. Predicted molecular weight: 56 kDa.|
FunctionMay be involved in microtubule organization and stabilization.
Tissue specificityHighly expressed in brain tissues, including cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Lower expression in spinal chord.
Sequence similaritiesContains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
DomainB30.2 box contains a microtubule-binding site.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Nucleus. Cytoplasm. Cleavage furrow. Cell-cycle-dependent association with the centrosome. Colocalizes with a subpopulation of microtubules. Does not associates with microtubules during mitosis but reassociates with microtubules during cytokinesis. Localizes to the central portions of a small subset of microtubules in interphase cells and a subpopulation of microtubules in the cleavage furrow, not present in the mitotic spindle.
- Information by UniProt
- Fibronectin type 3 and SPRY (spla, ryanodine) domain containing (with coiled-coil motif) 1 antibody
- Fibronectin type 3 and SPRY domain containing 1 antibody
- Fibronectin type III and SPRY domain containing 1 antibody
4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for FSD1 (green) using ab229666 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Paraffin-embedded mouse brain tissue stained for FSD1 using ab229666 at 1/1200 dilution in immunohistochemical analysis.
All lanes : Anti-FSD1 antibody (ab229666) at 1/1000 dilution
Lane 1 : U87-MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 (human neuroblast cell line) whole cell extract
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229666 has not yet been referenced specifically in any publications.