Recombinant Anti-FSH beta antibody [EPR24439-141] (ab281562)
![Western blot - Anti-FSH beta antibody [EPR24439-141] (ab281562)](https://www.abcam.com/ps/products/281/ab281562/Images/ab281562-458944-anti-fsh-beta-antibody-epr24439141-western-blot-lysate.jpg "Western blot - Anti-FSH beta antibody [EPR24439-141] (ab281562)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24439-141] to FSH beta
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FSH beta antibody [EPR24439-141]
See all FSH beta primary antibodies -
Description
Rabbit monoclonal [EPR24439-141] to FSH beta -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Fr, WBmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human pituitary, Mouse pituitary, Rat pituitary lysates. IHC-P: Human pituitary, Mouse pituitary, Rat pituitary tissues. IHC-Fr: Rat pituitary tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24439-141 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IHC-Fr |
1/100.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
| WB |
1/1000. Predicted molecular weight: 15 kDa.
|
| Notes |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
WB
1/1000. Predicted molecular weight: 15 kDa. |
Target
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Relevance
Follicle stimulating hormone enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell proliferation and maintenance of sperm quality in the testis. Members of the pituitary glycoprotein hormone family, of which FSH is one consist of a shared alpha chain and a beta chain encoded by a separate gene. The FSH beta gene encodes the beta subunit of follicle stimulating hormone. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2488 Human
- Entrez Gene: 14308 Mouse
- Entrez Gene: 25447 Rat
- Omim: 136530 Human
- SwissProt: P01225 Human
- SwissProt: Q60687 Mouse
- SwissProt: P18427 Rat
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Alternative names
- Follicle Stimulating Hormone antibody
- Follicle stimulating hormone beta polypeptide antibody
- Follicle stimulating hormone beta subunit antibody
see all
Images
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Western blot - Anti-FSH beta antibody [EPR24439-141] (ab281562)All lanes : Anti-FSH beta antibody [EPR24439-141] (ab281562) at 1/1000 dilution
Lane 1 : Human pituitary tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : Human spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 15 kDa
Observed band size: 23,19 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 29090093, 11502795).
Negative control: heart, spleen (PMID:29090093).
Exposure time: 81 seconds
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![Western blot - Anti-FSH beta antibody [EPR24439-141] (ab281562)](https://www.abcam.com/ps/products/281/ab281562/Images/ab281562-458945-anti-fsh-beta-antibody-epr24439141-western-blot-lysate1.jpg)
Western blot - Anti-FSH beta antibody [EPR24439-141] (ab281562)All lanes : Anti-FSH beta antibody [EPR24439-141] (ab281562) at 1/1000 dilution
Lane 1 : Mouse pituitary tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat pituitary tissue lysate
Lane 5 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 15 kDa
Observed band size: 23,19 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 29090093, 11502795).
Negative control: heart, spleen (PMID:29090093).
Exposure time: Lanes 1-3:3 minutes; Lanes 4-5:26 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)](https://www.abcam.com/ps/products/281/ab281562/Images/ab281562-1-anti-fsh-beta-antibody-epr24439141-immunohistochemistry-human-pituitary.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)Immunohistochemical analysis of paraffin-embedded Human pituitary tissue labelling FSH beta with ab281562 at 1/2000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human pituitary (PMID: 8930609). The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)](https://www.abcam.com/ps/products/281/ab281562/Images/ab281562-2-anti-fsh-beta-antibody-epr24439141-immunohistochemistry-mouse-pituitary.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)Immunohistochemical analysis of paraffin-embedded Mouse pituitary tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse pituitary. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)](https://www.abcam.com/ps/products/281/ab281562/Images/ab281562-3-anti-fsh-beta-antibody-epr24439141-immunohistochemistry-rat-pituitary.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)Immunohistochemical analysis of paraffin-embedded Rat pituitary tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat pituitary. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Frozen sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)](https://www.abcam.com/ps/products/281/ab281562/Images/ab281562-6-anti-fsh-beta-antibody-epr24439141-immunohistochemistry-frozen-rat-pituitary.jpg)
Immunohistochemistry (Frozen sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pituitary tissue labeling FSH beta with ab281562 at 1/100 dilution (4.75 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on rat pituitary is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)](https://www.abcam.com/ps/products/281/ab281562/Images/ab281562-4-anti-fsh-beta-antibody-epr24439141-immunohistochemistry-human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human spleen. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)](https://www.abcam.com/ps/products/281/ab281562/Images/ab281562-5-anti-fsh-beta-antibody-epr24439141-immunohistochemistry-human-cardiac-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human cardiac muscle. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-FSH beta antibody [EPR24439-141] (ab281562)](https://www.abcam.com/ps/products/281/ab281562/Images/ab281562-7-Benefits-of-Recombinant-antibodies.jpg)
Anti-FSH beta antibody [EPR24439-141] (ab281562)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab281562 has not yet been referenced specifically in any publications.
