Overview

  • Product name
    Anti-FSH beta antibody [INN-hFSH-60]
    See all FSH beta primary antibodies
  • Description
    Mouse monoclonal [INN-hFSH-60] to FSH beta
  • Host species
    Mouse
  • Specificity
    Ab11069 Shows strong reactions with hFSH and beta hFSH, but no cross reactivity with hTSH, hLH, hCG, alpha hCG, alpha hFSH. The recognition site is located near the alpha 1 binding site on the hFSH molecule. It is not compatible with other anti hFSH beta antibodies.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

  • Epitope
    Directed against the Beta 2 epitope of the Beta subunit of human FSH beta.
  • Positive control
    • Pituitary Gland.
  • General notes
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Follicle stimulating hormone enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell proliferation and maintenance of sperm quality in the testis. Members of the pituitary glycoprotein hormone family, of which FSH is one consist of a shared alpha chain and a beta chain encoded by a separate gene. The FSH beta gene encodes the beta subunit of follicle stimulating hormone.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Follicle Stimulating Hormone antibody
    • Follicle stimulating hormone beta polypeptide antibody
    • Follicle stimulating hormone beta subunit antibody
    • Follitropin beta chain antibody
    • Follitropin subunit beta antibody
    • FSH-B antibody
    • FSH-beta antibody
    • FSHB antibody
    • FSHbeta antibody
    see all

References

This product has been referenced in:
  • Jevdjovic T  et al. Insulin-like growth factor-I mRNA and peptide in the human anterior pituitary. J Neuroendocrinol 19:335-41 (2007). Read more (PubMed: 17425608) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

I am very sorry that the suggestions provided did not improve the performance of this product for you and wish to thank you for the time which you have put into troubleshooting this.

We would of course love to replace or refund this product for you. In order to do this I will need the Abcam order confirmation number or the PO number used to purchase this product. If you would like a replacement, please let me know whether you would prefer a new lot of ab105915, a new anti- COX IV product, perhaps ab14744 or another product.

I look forward to your reply. Please let me know if you have any questions.

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Answer

Thank you for this additional information.

I would like to offer additional suggestions based on this new information which may help you achieve good results using ab105915. When performing western blot on mitochondrial proteins there are a number of steps which our Mitosciences team suggest to create the best results.

For performing Western blotting transfer and detection, it is always recommended toisolate mitochondria from cells before analysis. Protocols for mitochondrial isolation can be found athttps://www.abcam.com/index.html?pageconfig=resource&rid=11411. While it is possible to probe whole tissueor cell extract, this may result in a weaker signal and/or additional bands resulting from non-specific cross-reactivity to non-mitochondrial proteins. Such as you may be seeing. Additionally we do offer mitochondria isolation kits for tissue (ab110169;https://www.abcam.com/Mitochondria-Isolation-Kit-for-Tissue-with-Dounce-Homogenizer-ab110169.htmland ab110168;https://www.abcam.com/Mitochondria-Isolation-Kit-for-Tissue-ab110168.html).



Samples should be solubilized in the SDS-PAGE loading buffer.After solubilization the sample should be spun at 13,000 rpm for 5 minutes. The supernatant should be collected and loaded onto the gel. Note: In most cases thesample need not be heated/boiled in this sample buffer prior to loading. Howeversample heating (95C for 5 min) may be incorporated into this protocol when non specific bands are present. Adding a reducing agent to the sample e.g. DTT, may also help.



I hope that this information is helpful and I look forward to hearing of your results with these suggestions.This product is covered by our Abpromise guarantee. If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, as you are using it according to specifications listed on our datasheet..Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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