Overview

  • Product name

  • Description

    Rabbit polyclonal to FSH-R
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other human proteins.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Pig, Monkey, Gorilla, Opossum, Common marmoset, House sparrow, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human FSH-R (N terminal).
    (Peptide available as ab210399)

  • Positive control

    • Human ovary tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab150557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 8 - 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
  • Tissue specificity

    Sertoli cells and ovarian granulosa cells.
  • Involvement in disease

    Defects in FSHR are a cause of ovarian dysgenesis type 1 (ODG1) [MIM:233300]; also known as premature ovarian failure or gonadal dysgenesis XX type or XX gonadal dysgenesis (XXGD) or hereditary hypergonadotropic ovarian failure or hypergonadotropic ovarian dysgenesis with normal karyotype. ODG1 is an autosomal recessive disease characterized by primary amenorrhea, variable development of secondary sex characteristics, and high serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH).
    Defects in FSHR are a cause of ovarian hyperstimulation syndrome (OHSS) [MIM:608115]. OHSS is a disorder which occurs either spontaneously or most often as an iatrogenic complication of ovarian stimulation treatments for in vitro fertilization. The clinical manifestations vary from abdominal distention and discomfort to potentially life-threatening, massive ovarian enlargement and capillary leak with fluid sequestration. Pathologic features of this syndrome include the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells, a condition called hyperreactio luteinalis.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications

    N-glycosylated; indirectly required for FSH-binding, possibly via a conformational change that allows high affinity binding of hormone.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Follicle stimulating hormone receptor antibody
    • Follicle stimulating hormone receptor isoform 1 antibody
    • Follicle-stimulating hormone receptor antibody
    • Follitropin receptor antibody
    • FSH receptor antibody
    • FSH-R antibody
    • Fshr antibody
    • FSHR_HUMAN antibody
    • FSHRO antibody
    • LGR1 antibody
    • MGC141667 antibody
    • MGC141668 antibody
    • ODG1 antibody
    • ovarian dysgenesis 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovary tissue, labeling FSH-R with ab150557 at 8 µg/ml.

References

This product has been referenced in:

  • Feng Z  et al. Expression of hypothalamic-pituitary-gonadal axis-related hormone receptors in low-grade serous ovarian cancer (LGSC). J Ovarian Res 10:7 (2017). Read more (PubMed: 28122595) »
  • Feng Z  et al. Hormone receptor expression profiles differ between primary and recurrent high-grade serous ovarian cancers. Oncotarget 8:32848-32855 (2017). Read more (PubMed: 28416763) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Flow Cytometry
Sample
Mouse Cell (testis cells)
Permeabilization
No
Gating Strategy
488-positive cells
Specification
testis cells
Preparation
Cell harvesting/tissue preparation method: enzymatic digestion of testis cells
Sample buffer: enriched PBS with 1% FBS 1% Pen/Strep, 1mg/ml glucose, 1mM pyruvate, 10 mM HEPES

Abcam user community

Verified customer

Submitted Feb 18 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (endometrium, myomerium)
Permeabilization
No
Specification
endometrium, myomerium
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 15 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric pH6 20min 100C
Permeabilization
Yes - proclin 300
Specification
testis
Blocking step
background buster from innovex as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2014

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