Anti-FSH-R antibody (ab75200)
Key features and details
- Rabbit polyclonal to FSH-R
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FSH-R antibody
See all FSH-R primary antibodies -
Description
Rabbit polyclonal to FSH-R -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human FSH-R (internal sequence).
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Positive control
- Transfected Jurkat and HUVEC cell extracts
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 78 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 78 kDa. |
Target
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Function
Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. -
Tissue specificity
Sertoli cells and ovarian granulosa cells. -
Involvement in disease
Defects in FSHR are a cause of ovarian dysgenesis type 1 (ODG1) [MIM:233300]; also known as premature ovarian failure or gonadal dysgenesis XX type or XX gonadal dysgenesis (XXGD) or hereditary hypergonadotropic ovarian failure or hypergonadotropic ovarian dysgenesis with normal karyotype. ODG1 is an autosomal recessive disease characterized by primary amenorrhea, variable development of secondary sex characteristics, and high serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH).
Defects in FSHR are a cause of ovarian hyperstimulation syndrome (OHSS) [MIM:608115]. OHSS is a disorder which occurs either spontaneously or most often as an iatrogenic complication of ovarian stimulation treatments for in vitro fertilization. The clinical manifestations vary from abdominal distention and discomfort to potentially life-threatening, massive ovarian enlargement and capillary leak with fluid sequestration. Pathologic features of this syndrome include the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells, a condition called hyperreactio luteinalis. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
Post-translational
modificationsN-glycosylated; indirectly required for FSH-binding, possibly via a conformational change that allows high affinity binding of hormone. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2492 Human
- Entrez Gene: 14309 Mouse
- Entrez Gene: 25449 Rat
- Omim: 136435 Human
- SwissProt: P23945 Human
- SwissProt: P35378 Mouse
- SwissProt: P20395 Rat
- Unigene: 1428 Human
see all -
Alternative names
- Follicle stimulating hormone receptor antibody
- Follicle stimulating hormone receptor isoform 1 antibody
- Follicle-stimulating hormone receptor antibody
see all
Images
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All lanes : Anti-FSH-R antibody (ab75200) at 1/500 dilution
Lane 1 : Transfected Jurkat cell extract
Lane 2 : Transfected HUVEC cell extract
Lane 3 : Transfected Jurkat cell extract with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab75200 has been referenced in 12 publications.
- Sheng S et al. Follicle-Stimulating Hormone Promotes the Development of Endometrial Cancer In Vitro and In Vivo. Int J Environ Res Public Health 19:N/A (2022). PubMed: 36430063
- Ebrahim N et al. Bone marrow-derived mesenchymal stem cells combined with gonadotropin therapy restore postnatal oogenesis of chemo-ablated ovaries in rats via enhancing very small embryonic-like stem cells. Stem Cell Res Ther 12:517 (2021). PubMed: 34579781
- Tao J et al. Effect of Exogenous Melatonin on the Development of Mice Ovarian Follicles and Follicular Angiogenesis. Int J Mol Sci 22:N/A (2021). PubMed: 34681919
- Hau RK et al. Generation of a hTERT-Immortalized Human Sertoli Cell Model to Study Transporter Dynamics at the Blood-Testis Barrier. Pharmaceutics 12:N/A (2020). PubMed: 33105674
- Zhang J et al. ACTL6A regulates follicle-stimulating hormone-driven glycolysis in ovarian cancer cells via PGK1. Cell Death Dis 10:811 (2019). PubMed: 31649264