Anti-FSP27 antibody (ab198204)
Key features and details
- Rabbit polyclonal to FSP27
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FSP27 antibody
See all FSP27 primary antibodies -
Description
Rabbit polyclonal to FSP27 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant full length protein corresponding to Human FSP27 aa 1 to the C-terminus. The identity of the protein fusion partner is GST.
Database link: Q96AQ7 -
Positive control
- Mouse intestinum tenue and heart tissue lysates; Human liver cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 27 kDa.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 27 kDa. |
Target
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Function
Induces apoptosis. -
Tissue specificity
Expressed mainly in small intestine, heart, colon and stomach and, at lower levels, in brain, kidney and liver. -
Sequence similarities
Contains 1 CIDE-N domain. -
Cellular localization
Cytoplasm. Cytoplasmic in a punctate manner. - Information by UniProt
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Database links
- Entrez Gene: 63924 Human
- Entrez Gene: 14311 Mouse
- Entrez Gene: 500292 Rat
- Omim: 612120 Human
- SwissProt: Q96AQ7 Human
- SwissProt: P56198 Mouse
- SwissProt: Q5XI33 Rat
- Unigene: 567562 Human
see all -
Alternative names
- Cell death activator antibody
- Cell death activator CIDE-3 antibody
- Cell death inducing DFFA like effector C antibody
see all
Images
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All lanes : Anti-FSP27 antibody (ab198204) at 1/551 dilution
Lane 1 : Mouse intestinum tenue tissue lysate
Lane 2 : Mouse heart tissue lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 27 kDa
Exposure time: 1 minute8% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling FSP27 with ab198204 at a 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab198204 has been referenced in 7 publications.
- Gupta A et al. Human CIDEC transgene improves lipid metabolism and protects against high-fat diet-induced glucose intolerance in mice. J Biol Chem 298:102347 (2022). PubMed: 35963433
- Wang CH et al. Losartan Prevents Hepatic Steatosis and Macrophage Polarization by Inhibiting HIF-1α in a Murine Model of NAFLD. Int J Mol Sci 22:N/A (2021). PubMed: 34360607
- Liu HM et al. Losartan Attenuates Insulin Resistance and Regulates Browning Phenomenon of White Adipose Tissue in ob/ob Mice. Curr Issues Mol Biol 43:1828-1843 (2021). PubMed: 34889901
- Lv H et al. Cell death-inducing DNA fragmentation factor-a-like effector C (CIDEC) regulates acetate- and ß-hydroxybutyrate-induced milk fat synthesis by increasing FASN expression in mammary epithelial cells of dairy cows. J Dairy Sci 104:6212-6221 (2021). PubMed: 33663853
- Hu J et al. Bariatric Surgery in Rats Upregulates FSP27 Expression in Fat Tissue to Affect Fat Hydrolysis and Metabolism. Biomed Res Int 2019:6415732 (2019). PubMed: 31205943
- Sans A et al. The Differential Expression of Cide Family Members is Associated with Nafld Progression from Steatosis to Steatohepatitis. Sci Rep 9:7501 (2019). PubMed: 31097771
- Yang Y et al. The effects of cell death-inducing DNA fragmentation factor-a-like effector C (CIDEC) on milk lipid synthesis in mammary glands of dairy cows. J Dairy Sci 100:4014-4024 (2017). PubMed: 28284693