Product nameAnti-FSTL1 antibody
See all FSTL1 primary antibodies
DescriptionGoat polyclonal to FSTL1
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Cynomolgus monkey
- Human Placenta and Ovary lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab11805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human FSTL1 peptide (ab23213). The observed molecular weight corresponds to earlier findings in literature (Tanaka et al, Int Immunol. 1998 Sep;10(9):1305-14.|
FunctionMay modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
Tissue specificityOverexpressed in synovial tissues from rheumatoid arthritis.
Sequence similaritiesContains 2 EF-hand domains.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Contains 1 VWFC domain.
- Information by UniProt
- Anti FSTL1 antibody
- FLJ50214 antibody
- FLJ52277 antibody
Anti-FSTL1 antibody (ab11805) at 0.5 µg/ml + Human Placenta lysate (RIPA buffer, 35µg total protein per lane)
Predicted band size: 35 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
This product has been referenced in:
- Sundaram GM et al. EGF hijacks miR-198/FSTL1 wound-healing switch and steers a two-pronged pathway toward metastasis. J Exp Med 214:2889-2900 (2017). Read more (PubMed: 28827448) »
- Mouillet JF et al. The expression and post-transcriptional regulation of FSTL1 transcripts in placental trophoblasts. Placenta 36:1231-8 (2015). Read more (PubMed: 26386648) »