Key features and details
- Rabbit polyclonal to FSTL1/FRP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FSTL1/FRP antibody
See all FSTL1/FRP primary antibodies
DescriptionRabbit polyclonal to FSTL1/FRP
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Cynomolgus monkey, Orangutan
- IHC-P: Human pancreas tissue. WB: FSTL1/FRP overexpression HEK-293T cell lysate. ICC/IF: U-2 OS cells.
This product was previously labelled as FSTL1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionMay modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
Tissue specificityOverexpressed in synovial tissues from rheumatoid arthritis.
Sequence similaritiesContains 2 EF-hand domains.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Contains 1 VWFC domain.
- Information by UniProt
- Anti FSTL1 antibody
- FLJ50214 antibody
- FLJ52277 antibody
All lanes : Anti-FSTL1/FRP antibody (ab224496) at 0.4 µg
Lane 1 : U2OS cell lysate
Lane 2 : Caco-2 cell lysate. Low expression expected.
Predicted band size: 35 kDa
Paraffin-embedded human pancreas tissue stained for FSTL1/FRP using ab224496 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-FSTL1/FRP antibody (ab224496) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : FSTL1/FRP overepression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Predicted band size: 35 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for FSTL1/FRP (green) using ab224496 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224496 has not yet been referenced specifically in any publications.