Anti-FSTL1/FRP antibody (ab71548)

Reviews (1)Q&A (3)References (4)

Overview

  • Product name

    Anti-FSTL1/FRP antibody
    See all FSTL1/FRP primary antibodies

  • Description

    Rabbit polyclonal to FSTL1/FRP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to FSTL1/FRP (N terminal).

  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 19th February 2018. The following lots is/are still unpurified and still in stock as of 19th February 2018 - GR200980-7, GR200980-9, GR200980-10. Lot numbers other than GR200980-7, GR200980-9, GR200980-10 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

     This product was previously labelled as FSTL1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab71548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 35 kDa.
IHC-P 1/50 - 1/500.

Target

  • Function

    May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.

  • Tissue specificity

    Overexpressed in synovial tissues from rheumatoid arthritis.

  • Sequence similarities

    Contains 2 EF-hand domains.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
    Contains 1 VWFC domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Anti FSTL1 antibody
    • FLJ50214 antibody
    • FLJ52277 antibody
    • Follistatin like 1 antibody
    • Follistatin related protein antibody
    • Follistatin-like protein 1 antibody
    • Follistatin-related protein 1 antibody
    • FRP antibody
    • FSL1 antibody
    • FSTL1 antibody
    • FSTL1 antibody antibody
    • FSTL1_HUMAN antibody
    • Micro RNA 198 antibody
    • MIR198 antibody
    • miRNA198 included antibody
    • OCC 1 antibody
    • OCC1 antibody
    see all

References

This product has been referenced in:

  • Zhang M  et al. BMP-2 overexpression augments vascular smooth muscle cell motility by upregulating myosin Va via Erk signaling. Oxid Med Cell Longev 2014:294150 (2014). Read more (PubMed: 24790701) »
  • Yucel G  et al. State-dependent signaling by Cav1.2 regulates hair follicle stem cell function. Genes Dev 27:1217-22 (2013). Read more (PubMed: 23752588) »
See all 4 Publications for this product

Publishing research using ab71548? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-FSTL1/FRP antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Soleus)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Treatment
-in KHB +/-1.2nM insulin for 4 or 24hrs
Specification
Soleus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Dr. Naveen Sharma

Verified customer

Submitted May 16 2012

Question

Thank you very much for the quick reply. Yes, we would like to receive this antibody ab107431 instead.

Read More
Answer

Thank you for confirming these details and for your cooperation.

As requested, I have issued a free of charge replacement with the order number #######.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Question

could you suggest another FSTL-1 antibody that works well in WB for you and we could try?

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Answer

Thank you for your reply.

Unfortunately it won't be possible to send the replacement to your collaborator.

What about ab107431?This rabbit polyclonal antibody is directed against the C-terminal part of Human FSTL1 and the immunogen sequence shows 83% homology with Rat and Mouse FSTL1. The link to the datasheet is https://www.abcam.com/ab107431.

Let me know if you would like to receive this antibody.

Read More

Question

Hello,

Our lab recently bought the anti-FSTL-1 Antibody (ab71548; order # 1021616). However we got a multiple bands for human, rat and mouse samples at different concentrations of proteins.

We blocked the membraine with 5% milk/TBST, 1:1000 anti-FSTL-1 (ab71548) 4 degree overnight, and 1:5000 anti-rabbit IgG HRP for one hour at room temperature.

I looked up the abcam website and saw another anti-fstl-1 antibody and was wondering if you can exchange ours with this one.

Thank you very much

Read More
Answer

Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem.

I have looked through your protocol, which looks fine to me. I appreciate the time that has been spent on these experiments and would be pleased to arrange a replacement with an alternative FSTL1 antibody. The link to the list is : https://www.abcam.com/index.html?t=72037&pt=1

I look forward to hearing from you with the reference catalog number of the antibody you would like to receive as replacement.

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