Recombinant
RabMAb

Recombinant Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961)

Overview

  • Product name

    Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free
    See all FSTL1/FRP primary antibodies
  • Description

    Rabbit monoclonal [EPR21933-155] to FSTL1/FRP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Rat FSTL1/FRP aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q62632

  • Positive control

    • WB: Rat and mouse E14 embryo lysate; C6 cell lysate.
  • General notes

    ab234961 is the carrier-free version of ab223287 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab234961 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as FSTL1

Properties

Applications

Our Abpromise guarantee covers the use of ab234961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
  • Tissue specificity

    Overexpressed in synovial tissues from rheumatoid arthritis.
  • Sequence similarities

    Contains 2 EF-hand domains.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
    Contains 1 VWFC domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Anti FSTL1 antibody
    • FLJ50214 antibody
    • FLJ52277 antibody
    • Follistatin like 1 antibody
    • Follistatin related protein antibody
    • Follistatin-like protein 1 antibody
    • Follistatin-related protein 1 antibody
    • FRP antibody
    • FSL1 antibody
    • FSTL1 antibody
    • FSTL1 antibody antibody
    • FSTL1_HUMAN antibody
    • Micro RNA 198 antibody
    • MIR198 antibody
    • miRNA198 included antibody
    • OCC 1 antibody
    • OCC1 antibody
    see all

Images

  • FSTL1/FRP was immunoprecipitated from 0.35 mg rat embryo lysate with ab223287 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223287 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: Rat embryo lysate 10 μg (Input).
    Lane 2: ab223287 IP in rat embryo lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223287 in rat embryo lysate (-).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 minute.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223287).

  • All lanes : Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287) at 1/1000 dilution

    Lane 1 : Rat E14 embryo lysate
    Lane 2 : Mouse E14 embryo lysate
    Lane 3 : C6 (Rat glial tumor cell line) cell lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 43-50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).

    Negative control: Rat liver (PMID: 20861081).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223287).

References

ab234961 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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