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8 nd ICC-IF, mouse samples
Can we use ab94482 and ab106386 in IHC-Fr, IHC-P and ICC-IF?
Asked on May 29 2012
rll yesterday afternoon. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
I would like to reassure you that ab92821 is tested and covered by our 6 month guarantee forICC-IF and mouse. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.
I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. I can suggest to provide just the information regarding ICC-IF to begin with, as this is covered by the guarantee. As discussed on the telephone, I am sorry to confirm that this antibody is not tested and guaranteed for IHC-P or IHC-Fr. All tested and guaranteed applications and species are listed on the online datasheets.
I would appreciate if you could also provide an image which would help us to assess the results.
With regards to ab94482 and ab106386:
ab94482 is not tested in IHC-Fr, IHC-P and ICC-IF. It has been tested on mouse.
ab106386 is tested in IHC-P only and I am sorry this is not tested in ICC-IF, IHC-Fr or on mouse samples. I have checked the alignment of the humanimmunogen with the mouse protein and this is86%. This is high,so theoreticallythe antibody islikely to detect in mouse. But please note this is not guaranteed because not tested.
I can offer a discount off a future purchase if you buy ab94482and want to testit inin IHC-Fr, IHC-P or ICC-IF. I can also offer a discount of a future purchase in you purchase ab106383 to test in mouse and in IHC-Fror ICC-IF.
I would suggest to wait until the resolution of this case before deciding how you would like to proceed with the discounts. However, I am pleased to provide information on how the discount works.
If you purchase ab94482 or ab106383 to test, then try the antibody and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody of the next order after submission of the Abreview. If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed. I will then send a discount code. This code must be issued before purchasing the antibodies so please wait for my reply before ordering. One code will be issued for each antibody.
2. Purchase the antibodieseither by phone, fax, or online (www.abcam.com).
3. Test the antibody in the species and applications discussed above.
ab94482 testin IHC-Fr, IHC-P or ICC-IF.
ab106383 test in mouse and in IHC-Fror ICC-IF.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody] ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for the species and application you are testing, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.
Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.
Purchase order number
or preferably Abcam order number:
Antibody storage conditions (temperature/reconstitution etc)
Description of the problem (high background, low signal, non-specific satining etc.)
Sample (Species/Tissue/Cell Type/Cell Line etc.)
Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)
Antigen retrieval (Enzymatic method, Heat mediated technique etc.)
Blocking conditions (Buffer/time period, Blocking agent etc.)
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Positive and negative controls used (please specify)
Optimization attempts (problem solving)
How many times have you tried the IHC?
Have you run a "No Primary" control?
Do you obtain the same results every time?
What steps have you altered?
We would appreciate if you are also able to provide and image which would help us to assess the results
Answered on May 29 2012