• Product name
  • Description
    Goat polyclonal to FTO
  • Host species
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 464-477 of Mouse FTO (NP_036066.2).

  • Positive control
    • Human Pancreas lysate.



Our Abpromise guarantee covers the use of ab77547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/128000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
    • Tissue specificity
      Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
    • Involvement in disease
      Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
    • Sequence similarities
      Belongs to the fto family.
    • Domain
      The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • AlkB homolog 9 antibody
      • ALKBH9 antibody
      • Alpha-ketoglutarate-dependent dioxygenase FTO antibody
      • AW743446 antibody
      • Fat mass and obesity-associated protein antibody
      • FATSO, MOUSE, HOMOLOG OF antibody
      • Fto antibody
      • FTO_HUMAN antibody
      • GDFD antibody
      • KIAA1752 antibody
      • mKIAA1752 antibody
      • Protein fatso antibody
      see all


    • Anti-FTO antibody (ab77547) at 0.03 µg/ml + Human Pancreas lysate (in RIPA buffer) at 35 µg

      Developed using the ECL technique.

      Predicted band size: 58 kDa
      Observed band size: 58 kDa

      Primary incubation was 1 hour.


    ab77547 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting us. The antibody was blocked in 3% milk in TBST for 1 hour at room temperature.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Thank you for contacting Abcam.

    For ab77547 we recommend blocking in 3% milk, along with incubating both the primary and secondary antibody in 3% milk also.

    Please let us know if there is anything else I can help you with.


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