Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mel-624)
Permeabilization
Yes - 0.05% Triton
Specification
Mel-624
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Fange Liu
Verified customer
Submitted Feb 08 2017